Mechanical Trap Surface-Enhanced Raman Spectroscopy for Three-Dimensional Surface Molecular Imaging of Single Live Cells
| Autor: | Beril Polat, Qianru Jin, Ming Li, Amy Zhang, Aimee Dai, Santosh Kumar Paidi, Ishan Barman, David H. Gracias, Jayson V. Pagaduan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Surface Properties Nanotechnology Breast Neoplasms 02 engineering and technology 010402 general chemistry Spectrum Analysis Raman 01 natural sciences Catalysis Cell membrane symbols.namesake Cell Line Tumor medicine Humans Multiplex Particle Size Plasmon General Chemistry Surface-enhanced Raman spectroscopy 021001 nanoscience & nanotechnology 0104 chemical sciences Molecular Imaging Membrane medicine.anatomical_structure symbols Surface modification Female Molecular imaging Single-Cell Analysis 0210 nano-technology Raman spectroscopy |
| Zdroj: | Angewandte Chemie (International ed. in English). 56(14) |
| ISSN: | 1521-3773 |
| Popis: | Reported is a new shell-based spectroscopic platform, named mechanical trap surface-enhanced Raman spectroscopy (MTSERS), for simultaneous capture, profiling, and 3D microscopic mapping of the intrinsic molecular signatures on the membrane of single live cells. By leveraging the functionalization of the inner surfaces of the MTs with plasmonic gold nanostars, and conformal contact of the cell membrane, MTSERS permits excellent signal enhancement, reliably detects molecular signatures, and allows non-perturbative, multiplex 3D surface imaging of analytes, such as lipids and proteins on the surface of single cells. The demonstrated ability underscores the potential of MTSERS to perform 3D spectroscopic microimaging and to furnish biologically interpretable, quantitative, and dynamic molecular maps in live cell populations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text