PDGF-B: The missing piece in the mosaic of PDGF family role in craniofacial development
| Autor: | Valentina Naef, Alessia Cericola, Michela Ori, Kety Giannetti, Debora Corsinovi |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Embryo Nonmammalian Morpholino cadherins cdh11 cdh6 cell migration cranial cartilages cranial nerves neural crest cells Xenopus laevis Xenopus Receptor tyrosine kinase Facial Bones 03 medical and health sciences Mice 0302 clinical medicine Cell Movement Cell Adhesion Animals Craniofacial biology Cadherin Skull Neural crest Cell migration Proto-Oncogene Proteins c-sis biology.organism_classification Cell biology 030104 developmental biology Neural Crest embryonic structures biology.protein 030217 neurology & neurosurgery Platelet-derived growth factor receptor Developmental Biology Signal Transduction |
| Popis: | BACKGROUND The platelet-derived growth factor (PDGF) family consists of four ligands (PDGF-A, PDGF-B, PDGF-C, PDGF-D) and two tyrosine kinase receptors (PDGFR-α and PDGFR-β). In vertebrates, PDGF signaling influences cell proliferation, migration, and matrix deposition, and its up-regulation is implicated in cancer progression. Despite this evidence, the role of each family member during embryogenesis is still incomplete and partially controversial. In particular, study of the role of pdgf signaling during craniofacial development has been focused on pdgf-a, while the role of pdgf-b is almost unknown due to the lethal phenotypes of pdgf-b-null mice. RESULTS By using a pdgf-b splice-blocking morpholino approach, we highlighted impairment of neural crest cell (NCC) migration in Xenopus laevis morphants, leading to alteration of NCC derivatives formation, such as cranial nerves and cartilages. We also uncovered a possible link between pdgf-b and the expression of cadherin superfamily members cdh6 and cdh11, which mediate cell-cell adhesion promoting NCC migration. CONCLUSIONS Our results suggested that pdgf-b signaling is involved in cranial NCC migration and it is required for proper formation of craniofacial NCC derivatives. Taken together, these data unveiled a new role for pdgf-b during vertebrate development, contributing to complete the picture of pdgf signaling role in craniofacial development. |
| Databáze: | OpenAIRE |
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