Systemic Signaling of the Plant Nitrogen Status Triggers Specific Transcriptome Responses Depending on the Nitrogen Source in Medicago truncatula
| Autor: | Marc Lepetit, Michael K. Udvardi, Christophe Salon, Marie Laure Martin-Magniette, Klementina Kakar, Alain Gojon, Jérôme Gouzy, Margaretha J. van der Merwe, Sandra Freixes, Sandrine Ruffel, Christian Jeudy, Pascal Tillard, Alisdair R. Fernie, Sandrine Balzergue |
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| Přispěvatelé: | Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Génétique et Ecophysiologie des Légumineuses à Graines (UMRLEG) (UMR 102), Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité de recherche en génomique végétale (URGV), Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Recherche Agronomique (INRA), Mathématiques et Informatique Appliquées (MIA-Paris), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Max-Planck-Institut für Molekulare Pflanzenphysiologie (MPI-MP), Max-Planck-Gesellschaft, Laboratoire des interactions plantes micro-organismes (LIPM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
| Rok vydání: | 2008 |
| Předmět: |
inorganic chemicals
0106 biological sciences Transcription Genetic Nitrogen Physiology Azote Functional response Plant Science Plant Roots 01 natural sciences Transcriptome 03 medical and health sciences Symbiosis Botany Gene expression Medicago Genetics [SDV.BV]Life Sciences [q-bio]/Vegetal Biology RNA Messenger Gene Root N Acquisiton 030304 developmental biology Regulator gene 2. Zero hunger 0303 health sciences biology Medicago Truncatula ACL fungi Nitrogen Source food and beverages biology.organism_classification Medicago truncatula Cell biology Split Root Rhizobium Genome Plant Signal Transduction Research Article 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2008, 146 (4), pp.2020-35. ⟨10.1104/pp.107.115667⟩ Plant Physiology, American Society of Plant Biologists, 2008, 146 (4), pp.2020-2035. ⟨10.1104/pp.107.115667⟩ Plant Physiology, 2008, 146 (4), pp.2020-2035. ⟨10.1104/pp.107.115667⟩ |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.107.115667 |
| Popis: | Legumes can acquire nitrogen (N) from NO3 −, NH4 +, and N2 (through symbiosis with Rhizobium bacteria); however, the mechanisms by which uptake and assimilation of these N forms are coordinately regulated to match the N demand of the plant are currently unknown. Here, we find by use of the split-root approach in Medicago truncatula plants that NO3 − uptake, NH4 + uptake, and N2 fixation are under general control by systemic signaling of plant N status. Indeed, irrespective of the nature of the N source, N acquisition by one side of the root system is repressed by high N supply to the other side. Transcriptome analysis facilitated the identification of over 3,000 genes that were regulated by systemic signaling of the plant N status. However, detailed scrutiny of the data revealed that the observation of differential gene expression was highly dependent on the N source. Localized N starvation results, in the unstarved roots of the same plant, in a strong compensatory up-regulation of NO3 − uptake but not of either NH4 + uptake or N2 fixation. This indicates that the three N acquisition pathways do not always respond similarly to a change in plant N status. When taken together, these data indicate that although systemic signals of N status control root N acquisition, the regulatory gene networks targeted by these signals, as well as the functional response of the N acquisition systems, are predominantly determined by the nature of the N source. |
| Databáze: | OpenAIRE |
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