Gradation of proteins and cells attached to the surface of bio-inert zwitterionic polymer brush
| Autor: | Hiromi Kitano, Kohji Ohno, Lifu Li, Takahiro Kishioka, Tadashi Nakaji-Hirabayashi, Yuki Usui |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Polymers
Surface Properties Ultraviolet Rays Biocompatible Materials 02 engineering and technology 010402 general chemistry Polymer brush Photochemistry 01 natural sciences Fluorescence Polymerization chemistry.chemical_compound Mice Adsorption Colloid and Surface Chemistry Monolayer Polymer chemistry Cell Adhesion Animals Physical and Theoretical Chemistry Atom-transfer radical-polymerization Photoelectron Spectroscopy Substrate (chemistry) Serum Albumin Bovine General Medicine Surfaces and Interfaces 021001 nanoscience & nanotechnology 0104 chemical sciences Betaine Monomer chemistry NIH 3T3 Cells Cattle 0210 nano-technology Protein adsorption Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 144:180-187 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2016.04.005 |
| Popis: | A self-assembled monolayer (SAM) of a 2-bromoisobutyryl end group-carrying initiator for atom transfer radical polymerization (ATRP) was constructed on the surface of silicon wafer or glass substrates via a silane-coupling reaction. When the initiator SAM was irradiated with UV light at 254nm, the surface density of bromine atoms was reduced by the scission of CBr bonds as observed by XPS. With the surface-initiated ATRP of the zwitterionic vinyl monomer, carboxymethyl betaine (CMB), the surface density of PCMB brushes could be easily varied by changing the irradiation period of UV light prior to the polymerization. Furthermore, by using a UV-cut shutter sliding above the initiator SAM-modified substrate at a constant speed, the degree of bromine atom removal could be linearly varied along the direction of movement of the shutter. Consequently, the amount of both proteins adsorbed and cells adhered to the PCMB brush-covered substrate could easily be controlled by the gradation of the surface density of PCMB brushes, which suppressed protein adsorption and cell adhesion. Such a technique is very simple and useful for the regulation of the surface density of adsorbed proteins and adhered cells on an originally bio-inert surface. |
| Databáze: | OpenAIRE |
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