Visualization of West Nile Virus in Urine Sediment using Electron Microscopy and Immunogold up to Nine Years Postinfection
| Autor: | Amber S. Podoll, Kristy O. Murray, Shannon E. Ronca, Patricia Navarro, Melissa S. Nolan, Rodion Gorchakov, Kevin W. Finkel, Sreedhar Mandayam, Steven Kolodziej |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine viruses Urine Biology Kidney Virus Cohort Studies 03 medical and health sciences Viral envelope Risk Factors Virology Prevalence medicine Humans Renal Insufficiency Chronic virus diseases Articles Immunogold labelling Middle Aged biology.organism_classification medicine.disease Texas nervous system diseases Microscopy Electron Flavivirus 030104 developmental biology Infectious Diseases medicine.anatomical_structure Renal pathology Female Parasitology West Nile virus West Nile Fever Kidney disease |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene. 97:1913-1919 |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.17-0405 |
| Popis: | West Nile virus (WNV) is an important emerging flavivirus in North America. Experimental studies in animals infer the development of persistent infection in the kidneys. In humans, recent studies suggest the possibility of persistent renal infection and chronic kidney disease. Considering the discrepancies between published studies on viral RNA detection in urine of convalescing WNV-positive patients, we explored the use of electron microscopy (EM) with anti-WNV E protein antibody immunogold labeling to detect virus in the urine sediment from a subset of study participants in the Houston WNV cohort. In 42% of evaluated study participants had visible sediment present in urine after centrifugation; viral particles consistent with the size and morphology of WNV were successfully detected using EM in the urine sediment up to 9 years postinfection. The anti-WNV immunogold labeling bound to virus envelope in the sediment allowed for enhanced detection when compared with PCR and provide a new technique for understanding kidney disease in WNV patients. These results provide further evidence of persistent infection in at least a subset of individuals infected with WNV. These findings present a novel tool to diagnose persistent WNV infection and its possible link with progressive renal pathology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|