Immunocytochemical localization of an allatotropin in developmental stages of Heliothis virescens and Apis mellifera
| Autor: | Anna Rachinsky, Julie M. Glasscock, Akira Mizoguchi |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
animal structures Physiology Neuropeptide Moths Internal medicine medicine Animals Larva biology Heliothis virescens Neuropeptides fungi Brain Bees biology.organism_classification Immunohistochemistry Ganglia Invertebrate Ganglion Cell biology Endocrinology medicine.anatomical_structure Manduca sexta Insect Hormones Insect Science Instar Corpus allatum |
| Zdroj: | Journal of Insect Physiology. 51:345-355 |
| ISSN: | 0022-1910 |
| DOI: | 10.1016/j.jinsphys.2004.12.005 |
| Popis: | Juvenile hormone biosynthesis by the corpora allata is regulated by stimulatory neuropeptides called allatotropins and inhibitory neuropeptides called allatostatins. This study localized Manduca sexta allatotropin-like material in developmental stages of the noctuid moth Heliothis virescens and the honeybee Apis mellifera. Immunocytochemical methods using both fluorescence-tagged antibodies and enzyme-coupled antibodies were used to stain the central nervous tissue of both species. H. virescens contains M. sexta allatotropin (Manse-AT)-like material consistently throughout larval development. The distribution patterns of Manse-AT immunoreactive cell bodies in the CNS persisted from one larval instar to the next. It will be discussed how larval Manse-AT distribution patterns differed from those in adults. The total number of AT-containing cells in brain and subesophageal ganglion gradually increased during larval development, whereas in the thoracic and abdominal ganglia, the number of AT-containing neurons remained constant. In the honeybee A. mellifera, Manse-AT immunoreactive cells were only found in a few brains from late last instar larvae (prepupae). Manse-AT-like material was present in a group of 6-8 cells in the pars intercerebralis. However, we did not find any Manse-AT-like material in brains of early last instar larvae, whose corpora allata (CA) are more sensitive to in vitro stimulation by Manse-AT than prepupal CA. |
| Databáze: | OpenAIRE |
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