Ca2+binding of latent transforming growth factor-β1 binding protein
| Autor: | Kohei Miyazono, Ulf Hellman, Carl-Henrik Heldin, Pascal Colosetti |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Protein Conformation
medicine.medical_treatment Proteolysis Biophysics Biochemistry Cell Line law.invention Transforming Growth Factor beta Structural Biology law Binding protein Genetics medicine Humans Platelet Binding site Molecular Biology medicine.diagnostic_test Ca2+ binding Chemistry Growth factor Intracellular Signaling Peptides and Proteins Cell Biology Recombinant Proteins In vitro Transforming growth factorβ Latent TGF-beta Binding Proteins EGF-like repeat Recombinant DNA Calcium Carrier Proteins Transforming growth factor |
| Zdroj: | FEBS Letters. 320:140-144 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(93)80079-a |
| Popis: | Latent transforming growth factor-beta 1 binding protein (LTBP) is a constituent of the latent high molecular weight complex of TGF-beta 1 in human platelets. In the present communication, we show that LTBP binds Ca2+ in its free form as well as in the latent TGF-beta 1 complex. The binding of Ca2+ induces a structural change which protects the molecule against proteolysis and changes its elution position when analyzed by anion exchange chromatography. The in vitro activation of TGF-beta 1 is not influenced by the presence of Ca2+. The possible significance of Ca2+ binding of LTBP is discussed. |
| Databáze: | OpenAIRE |
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