Fluid shear stress induces cancer stem cell-like phenotype in MCF7 breast cancer cell line without inducing epithelial to mesenchymal transition
| Autor: | Ursula L. Triantafillu, Andrew D. Raddatz, Yonghyun Kim, Nikki L. Klaassen, Seungjo Park |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Epithelial-Mesenchymal Transition Breast Neoplasms Biology medicine.disease_cause Metastasis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Circulating tumor cell Cancer stem cell Stress Physiological Cell Line Tumor medicine Biomarkers Tumor Humans Epithelial–mesenchymal transition Neoplasm Metastasis Cancer Epithelial cell adhesion molecule medicine.disease Neoplastic Cells Circulating Cell biology 030104 developmental biology Oncology chemistry 030220 oncology & carcinogenesis Cancer cell Hydrodynamics MCF-7 Cells Neoplastic Stem Cells Female Carcinogenesis |
| Zdroj: | International journal of oncology. 50(3) |
| ISSN: | 1791-2423 |
| Popis: | Metastasis is the leading cause of cancer deaths due to the spread of cancer cells through the blood vessels and the subsequent formation of secondary tumors. Metastasizing cancer cells in the human vasculature are called circulating tumor cells (CTCs) and are characterized to express the epithelial cell adhesion molecule (EpCAM). They are further known to survive physiological fluid shear stress (FSS) conditions. However, the effect of FSS on CTC molecular phenotype, such as the epithelial to mesenchymal transition (EMT) and cancer stem cell (CSC) expression, has not been extensively studied. Here, CTCs in FSS are evaluated in an in vitro model system. MCF7 and MDA-MB-231 breast cancer cell lines were grown in adherent and suspension culture media. The cell lines were tested for EMT and CSC genetic and protein markers using qRT-PCR and flow cytometry, respectively. Suspension cells showed a significantly increased EMT signature compared to adherent cells (p0.05), suggesting that they model cells detaching from primary tumors in vivo. Upon application of FSS, MCF7 and MDA-MB-231 cells did not show a significant change in EMT expression (p0.05), but there was a statistically significant increase of the CSC population in MCF7 suspension cultures (p0.05). These results with MCF7 suggest that CTCs can be modeled in vitro as non-adherent cancer cells in FSS and that they show an increased CSC-like signature during circulation, providing new insights to the importance of CSC-targeting strategies when treating metastatic patients. |
| Databáze: | OpenAIRE |
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