Phospholipid-based ultrasonic microbubbles for loading protein and ultrasound-triggered release

Autor:	Ying-Zheng Zhao, Zhicai Zhou, Cui-Tao Lu, Jiang Yina, Hui-Sheng Gao, Xiao-Kun Li, Ji-Lai Tian, Gang-Tao Zhao, Wei Yang, Hong-Xing Fu, Shu-Ping Hu
Rok vydání:	2009
Předmět:	Male Serum albumin Phospholipid Pharmaceutical Science chemistry.chemical_compound Drug Stability In vivo Drug Discovery Animals Ultrasonics Bovine serum albumin Particle Size Phospholipids Ultrasonography Pharmacology Drug Carriers Chromatography biology Organic Chemistry Proteins Serum Albumin Bovine Molecular Weight chemistry Liver biology.protein Microbubbles Liberation Particle size Rabbits Drug carrier
Zdroj:	Drug development and industrial pharmacy. 35(9)
ISSN:	1520-5762
Popis:	Background: Ultrasonic microbubbles are used as ultrasound-triggered delivery carriers for protein drugs. Aim: This work was to prepare stabilized protein-loaded phospholipid-based ultrasonic microbubbles (PUM) and to determine its value as a protein delivery system. Method: Bovine serum albumin (BSA) was used as a model protein drug. BSA-containing PUM were prepared by dissolving lyophilized PUM powder in BSA solution. The particle size and microbubble concentration of BSA-containing PUM were measured. The BSA encapsulation efficiency as a function of BSA concentration was determined. Contrast enhancement of BSA-containing PUM in vivo was detected. The release profile of BSA from PUM was also investigated. Results: The mean particle size and microbubble concentration of PUM were unchanged by the presence of BSA for at least 30 minutes after preparation. The net amount of BSA entrapped in PUM was maintained unchanged with increasing BSA concentration. BSA-containing PUM were shown easily to be visible in in vivo rabbit kidney. There was no difference in echogenicity between the loaded and unloaded PUM. Ultrasound duration had a positive relationship with BSA release. Ultrasound of 30 seconds stimulated 94.1% and 93.3% of BSA release from PUM solutions containing 0.3% and 1.5% BSA, respectively. Conclusions: Protein-loaded PUM exhibited satisfactory physical characteristics and were potent for using in ultrasound-triggered delivery.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20cffa2944a955bfe80a273ff7ced5e3 https://pubmed.ncbi.nlm.nih.gov/19555252 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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