Microfluidic devices for DNA sequencing: sample preparation and electrophoretic analysis
| Autor: | Robert G. Blazej, Brian M. Paegel, Richard A. Mathies |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Electrophoresis
Sequence analysis Molecular Sequence Data Microfluidics Biomedical Engineering Bioengineering Computational biology Biology Polymerase Chain Reaction DNA sequencing Specimen Handling law.invention law Nanotechnology Polymerase chain reaction Oligonucleotide Array Sequence Analysis Genetics Massive parallel sequencing Base Sequence Microchemistry DNA Equipment Design Robotics Sequence Analysis DNA Flow Cytometry Isolation (microbiology) Systems Integration DNA sequencer Human genome Rheology Biotechnology |
| Zdroj: | Current Opinion in Biotechnology. 14:42-50 |
| ISSN: | 0958-1669 |
| DOI: | 10.1016/s0958-1669(02)00004-6 |
| Popis: | Modern DNA sequencing 'factories' have revolutionized biology by completing the human genome sequence, but in the race to completion we are left with inefficient, cumbersome, and costly macroscale processes and supporting facilities. During the same period, microfabricated DNA sequencing, sample processing and analysis devices have advanced rapidly toward the goal of a 'sequencing lab-on-a-chip'. Integrated microfluidic processing dramatically reduces analysis time and reagent consumption, and eliminates costly and unreliable macroscale robotics and laboratory apparatus. A microfabricated device for high-throughput DNA sequencing that couples clone isolation, template amplification, Sanger extension, purification, and electrophoretic analysis in a single microfluidic circuit is now attainable. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect