DDX50 inhibits the replication of dengue virus 2 by upregulating IFN-β production
| Autor: | Yingfeng Lei, Linfeng Cheng, Hongwei Ma, Zhikai Xu, Peijun Han, Hesong Chen, Wei Ye, Yangchao Dong, Liang Zhang, Fanglin Zhang, Xin Lv, Daihui Weng |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Replication Interferon-Induced Helicase IFIH1 medicine.drug_class viruses Dengue virus Biology medicine.disease_cause Virus Replication Cell Line DEAD-box RNA Helicases 03 medical and health sciences Immune system Transcription (biology) Virology medicine Humans Antibody-dependent enhancement Receptors Immunologic Gene 030102 biochemistry & molecular biology DNA replication virus diseases General Medicine Interferon-beta biochemical phenomena metabolism and nutrition Dengue Virus Immunity Innate Up-Regulation 030104 developmental biology HEK293 Cells Viral replication Gene Expression Regulation DEAD Box Protein 58 Antiviral drug |
| Zdroj: | Archives of virology. 162(6) |
| ISSN: | 1432-8798 |
| Popis: | Dengue virus (DENV) infects approximately 390 million people per year, and each of the four DENV serotypes (DENV-1, DENV-2, DENV-3, and DENV-4) is capable of causing infection. At present, there is no antiviral drug available for the treatment of DENV. Several DExD/H-box helicases have been shown to be involved in the antiviral immune response or viral replication. In the present study, we investigated the role of DDX50 in DENV-2 RNA replication. Our data showed that the level of DENV-2 RNA increased in DDX50 knockdown cells during an early stage of viral infection and decreased in DDX50-overexpressing cells. DDX50, in conjunction with RIG-I and MDA5, upregulated the production of IFN-β in infected cells through an additive effect on the IFN-β promoter. Furthermore, transcription of several IFN-stimulated genes was increased in DDX50-overexpressing cells infected with DENV-2. These results provide evidence that DDX50 negatively regulates DENV-2 replication during the early stages of infection by inducing IFN-β production. |
| Databáze: | OpenAIRE |
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