Advances in cryopreservation of stallion semen in modified INRA82

Autor: G Arnaud, A Le Tellier, I Couty, J. M. Yvon, J Nguekam-Feugang, F Noel, S Cottron, Marianne Vidament, Michèle Magistrini, P Noue, Eric Palmer
Přispěvatelé: Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration
Rok vydání: 2001
Předmět:
Zdroj: Animal Reproduction Science
Animal Reproduction Science, Elsevier Masson, 2001, 68, pp.201-218
3. International Symposium on Stallion Reproduction
3. International Symposium on Stallion Reproduction, Jan 2001, Fort Collins Colorado, United States
ISSN: 0378-4320
DOI: 10.1016/s0378-4320(01)00157-9
Popis: In the procedure used in this paper, semen was first diluted in INRA82+2% egg yolk (E1) at 37 degrees C. Before or after cooling to 4 degrees C, semen was centrifuged and diluted in E1+2.5% glycerol (E2). Cooled semen was frozen in 0.5-ml straws. Straws were thawed at 37 degrees C for 30s. For fertility trials, frozen ejaculates were used only if total post-thaw motility was above 35%. Most mares were inseminated two times before ovulation with 400 x 10(6) total spermatozoa every 24h. This paper presents post-thaw motility (CASA) and fertility results obtained when some steps of the procedure were evaluated. Use of the first three jets of ejaculate before the centrifugation did not improve post-thaw motility compared to use of the whole semen (25% versus 25%, 2 stallions x 12 ejaculates, P0.80). When the first dilution was performed in E2 at 22 degrees C instead of in E1 at 37 degrees C, motility was slightly improved (38% versus 36%, n283 ejaculates per group, P0.04) but fertility was similar (51% versus 58%, n196 cycles per group, P0.10). Coating the spermatozoa with 0.5, 1, 2, 4 and 8mM of Concanavalin A resulted in unchanged post-thaw motility (6 stallions x 3 ejaculates, P0.05). The extender E2 was modified or supplemented with different substances. Increasing egg yolk concentration from 2 to 4% (v/v) did not increase post-thaw motility (42% versus 34%, 6 stallions x 2 ejaculates, P0.05). Different glycerol concentrations (range: 1.7-3.7%) had no significant effect on post-thaw motility even though 2.4-2.8% resulted in a nonsignificant higher motility (7 stallions x 2 ejaculates, P0.05). Glutamine at 50mM in E2 improved post-thaw motility compared with no glutamine (49% versus 46%, n584 ejaculates per group, P0.0001) but not fertility (53% versus 54%, n451 cycles per group, P0.80). Thawing at 75 degrees C for 10s slightly increased motility after 120 min at 37 degrees C (6 stallions x 1 ejaculate, P0.05) but no effect on per-cycle fertility was noted (32% (19 cycles) versus 41% (17 cycles), P0.50). When post-thaw dilution was performed using a fixed molarity multi-step system (25 mOsm per step) from various osmolarities (900-690 mOsm) to 365 mOsm, motility was unaffected compared with dilution in one step (36% versus 38%, 6 stallions x 1 ejaculate, P0.20).
Databáze: OpenAIRE
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