Gut Dysbiosis with Minimal Enteritis Induced by High Temperature and Humidity
Autor: | Qin Tang, Yiqing Zhou, Huanhuan Luo, Weiwei Hu, Guang-Li Rong, Weizhong Guo, Song Chen, Yuhua Zheng, Jianbang Tang |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Diarrhea
Male 0301 basic medicine Hot Temperature medicine.medical_treatment lcsh:Medicine Enzyme-Linked Immunosorbent Assay Biology Gut flora Article Microbiology Enteritis law.invention Pathogenesis Mice 03 medical and health sciences Probiotic 0302 clinical medicine law RNA Ribosomal 16S medicine Animals Microbiome Intestinal Mucosa lcsh:Science Inflammation Multidisciplinary Probiotics Body Weight Gastrointestinal Microbiome lcsh:R Humidity Chronic inflammation Organ Size medicine.disease biology.organism_classification Intestines Mice Inbred C57BL 030104 developmental biology Cytokine Bacterial Translocation 030220 oncology & carcinogenesis Dysbiosis lcsh:Q medicine.symptom Signal Transduction |
Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | High temperature and humidity (HTH) can cause diarrhea owing to food and drinking water contamination. However, their direct effects on gut microbiota and gastrointestinal inflammation are unknown. This study aimed to investigate the effects of HTH and probiotics on the microbiome. Twenty-one male mice were randomly assigned to normal control (NC), HTH, and broad-spectrum probiotic-treated (PR) groups. HTH and PR groups were regularly housed at 30 ± 0.5 °C with humidity of 85–90% for eight consecutive weeks. A broad-spectrum probiotic was administrated to PR-group mice from day 50 to 56. Clinical signs were observed and gut microbiota were analyzed via 16 S rRNA-based functional metagenomics. Intestinal pathology and the expression of defensins and pro-inflammatory cytokines were also assessed. Mice in the HTH and PR groups gradually developed sticky or loose feces. The HTH group developed a distinct microbiota profile associated with augmented metabolism and human-like pathophysiologies upon suppression of environmental sensing. Pathological assays indicated minimal enteritis, increased bacterial translocation, and elevated intestinal pro-inflammatory cytokine levels. Thus, ambient HTH directly contributes to gut dysbiosis and minimal enteritis, whereas probiotics partially normalized the microbiota and ameliorated gut inflammation. This study provides novel insights into the pathogenesis of environment-associated diseases and offers a potential therapeutic approach. |
Databáze: | OpenAIRE |
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