Activation of PI3 Kinase/Akt Signaling in Chronic Subdural Hematoma Outer Membranes
| Autor: | Masakazu Takayasu, Kimie Atsuzawa, Koji Osuka, Muneyoshi Yasuda, Mikiko Funai, Yasuo Watanabe, Takashi Inukai, Nobuteru Usuda |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Pathology medicine.medical_specialty Angiogenesis medicine.medical_treatment Pathogenesis chemistry.chemical_compound Meninges Enos medicine Humans Phosphatidylinositol Protein kinase B PI3K/AKT/mTOR pathway Aged biology Growth factor Middle Aged biology.organism_classification Vascular endothelial growth factor chemistry Hematoma Subdural Chronic Female Neurology (clinical) Phosphatidylinositol 3-Kinase Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Journal of Neurotrauma. 28:1127-1131 |
| ISSN: | 1557-9042 0897-7151 |
| Popis: | Chronic subdural hematoma (CSDH) is an angiogenic disease that is recognized as a cause of treatable dementia with unknown pathogenesis. Vascular endothelial growth factor (VEGF), a potent growth factor regulating angiogenesis through the phosphatidylinositol 3-kinase (PI3-kinase)/Akt pathway, has been implicated in its etiology. The status of this signaling pathway in CSDH outer membranes was examined in the present study, using outer membranes obtained during trepanation surgery. Expressions of PI3-kinase, PKB-kinase, Akt, phosphorylated Akt at Ser(473) (p-Akt), endothelial nitric oxide synthase (eNOS), vascular endothelial-cadherin (VE-cadherin), and actin were examined by Western blot analysis, together with their immunohistochemistry. PI3-kinase, Akt, eNOS, and VE-cadherin were detected in all cases. The magnitude of the expression of p-Akt varied among cases; however, the localization was revealed to be present in endothelial cells of vessels in CSDH outer membranes, together with VEGF and VE-cadherin detected in endothelial cells of vessels. These findings suggest that the PI3-kinase/Akt signaling is activated in CSDH outer membranes, and indicate the possibility that the PI3 kinase/Akt pathway might be activated by VEGF and play a critical role in the angiogenesis of CSDH. |
| Databáze: | OpenAIRE |
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