An adenovirus vectored mucosal adjuvant augments protection of mice immunized intranasally with an adenovirus-vectored foot-and-mouth disease virus subunit vaccine

Autor: Marvin J. Grubman, Edan R. Tulman, Diana M. Alejo, Debra Rood, X. Liao, Fayna Diaz-San Segundo, Lawrence K. Silbart, Mauro P. Moraes, Camila C. Dias
Rok vydání: 2013
Předmět:
Serotype
Swine
animal diseases
viruses
medicine.medical_treatment
Bacterial Toxins
Genetic Vectors
Biology
Antibodies
Viral
Virus
Adenoviridae
Cell Line
Enterotoxins
Mice
Immune system
Adjuvants
Immunologic
Immunity
Escherichia coli
medicine
Animals
Viremia
Immunity
Mucosal
Administration
Intranasal
General Veterinary
General Immunology and Microbiology
Escherichia coli Proteins
Public Health
Environmental and Occupational Health
Viral Vaccines
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Antibodies
Neutralizing
Virology
Immunity
Humoral
Mice
Inbred C57BL
Infectious Diseases
Immunoglobulin M
Foot-and-Mouth Disease Virus
Foot-and-Mouth Disease
Immunoglobulin G
Vaccines
Subunit
Immunology
biology.protein
Molecular Medicine
Female
Nasal administration
Antibody
Foot-and-mouth disease virus
Adjuvant
Zdroj: Vaccine. 31:2302-2309
ISSN: 0264-410X
Popis: Foot-and-mouth disease virus (FMDV) is a highly contagious pathogen that causes severe morbidity and economic losses to the livestock industry in many countries. The oral and respiratory mucosae are the main ports of entry of FMDV, so the stimulation of local immunity in these tissues may help prevent initial infection and viral spread. E. coli heat-labile enterotoxin (LT) has been described as one of the few molecules that have adjuvant activity at mucosal surfaces. The objective of this study was to evaluate the efficacy of replication-defective adenovirus 5 (Ad5) vectors encoding either of two LT-based mucosal adjuvants, LTB or LTR72. These vectored adjuvants were delivered intranasally to mice concurrent with an Ad5-FMDV vaccine (Ad5-A24) to assess their ability to augment mucosal and systemic humoral immune responses to Ad5-A24 and protection against FMDV. Mice receiving Ad5-A24 plus Ad5-LTR72 had higher levels of mucosal and systemic neutralizing antibodies than those receiving Ad5-A24 alone or Ad5-A24 plus Ad5-LTB. The vaccine plus Ad5-LTR72 group also demonstrated 100% survival after intradermal challenge with a lethal dose of homologous FMDV serotype A24. These results suggest that Ad5-LTR72 could be used as an important tool to enhance mucosal and systemic immunity against FMDV and potentially other pathogens with a common route of entry.
Databáze: OpenAIRE
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