The antioxidant butylated hydroxyanisole potentiates the toxic effects of propylparaben in cultured mammalian cells
| Autor: | Paloma Fernández Freire, Miguel A. Lasunción, Lidia Daimiel, M.J. Hazen, José Manuel Pérez Martín, Javier Martínez-Botas, Ana Peropadre, Covadonga Martín Sánchez |
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| Rok vydání: | 2014 |
| Předmět: |
Antioxidant
Cell Survival Endpoint Determination DNA damage medicine.medical_treatment Butylated Hydroxyanisole Parabens Biology Toxicology medicine.disease_cause Antioxidants chemistry.chemical_compound Chlorocebus aethiops medicine Animals Viability assay Cytotoxicity Vero Cells Cell Proliferation Cell Cycle Checkpoints General Medicine Microarray Analysis Oxidative Stress chemistry Biochemistry Vero cell Butylated hydroxyanisole Reactive Oxygen Species Propylparaben Oxidative stress DNA Damage Food Science |
| Zdroj: | Food and Chemical Toxicology. 72:195-203 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2014.07.031 |
| Popis: | Butylated hydroxyanisole and propylparaben are phenolic preservatives commonly used in food, pharmaceutical and personal care products. Both chemicals have been subjected to extensive toxicological studies, due to the growing concern regarding their possible impacts on environmental and human health. However, the cytotoxicity and underlying mechanisms of co-exposure to these compounds have not been explored. In this study, a set of relevant cytotoxicity endpoints including cell viability and proliferation, oxidative stress, DNA damage and gene expression changes were analyzed to assess whether the antioxidant butylated hydroxyanisole could prevent the pro-oxidant effects caused by propylparaben in Vero cells. We demonstrated that binary mixtures of both chemicals induce greater cytotoxic effects than those reported after single exposureto each compound. Simultaneous treatment with butylated hydroxyanisole and propylparaben caused G0/G1 cell cycle arrest as a result of enhanced generation of oxidative stress and DNA double strand breaks. DNA microarray analysis revealed that a cross-talk between transforming growth factor beta (TGFβ) and ataxia-telangiectasia mutated kinase (ATM) pathways regulates the response of Vero cells to the tested compounds in binary mixture. Our findings indicate that butylated hydroxyanisole potentiates the pro-oxidant effects of propylparaben in cultured mammalian cells and provide useful information for their safety assessment. |
| Databáze: | OpenAIRE |
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