Immunogenicity of the Shigella flexneri serotype Y (SFL 124) vaccine strain expressing cloned glucosyl transferase gene of converting bacteriophage SfX
| Autor: | Naresh K. Verma, Alf A. Lindberg, Pham Thi Huan, Rona Taylor |
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| Rok vydání: | 1995 |
| Předmět: |
Serotype
DNA Bacterial Keratoconjunctivitis Infectious Male Immunology Guinea Pigs Molecular Sequence Data Transfection Microbiology Virus Gene Expression Regulation Enzymologic Shigella flexneri Bacteriophage Antigen Virology Animals Bacteriophages Serotyping Dysentery Bacillary Antigens Bacterial Vaccines Synthetic biology Strain (chemistry) Immunogenicity biology.organism_classification Enterobacteriaceae Carbohydrate Sequence Glucosyltransferases Bacterial Vaccines Immunization |
| Zdroj: | Microbiology and immunology. 39(7) |
| ISSN: | 0385-5600 |
| Popis: | The glucosyl transferase gene (gtr) from bacteriophage phage X (SfX) caused partial conversion of serotype Y (group antigen 3, 4) to X (group antigen 7, 8) when introduced into a candidate vaccine strain of Shigella flexneri serotype Y (SFL124). The gtr gene caused conversion of O-antigens but did not eliminate the adsorption of the corresponding phage SfX. The hybrid strain expressing both group antigens 7, 8 and 3, 4 showed 75% protection when immunized guinea pigs were challenged with a wild-type S. flexneri serotype X strain. No protection was observed against serotype Y challenge, although group antigen 3, 4 was detected in the LPS of the hybrid strain. This suggests the importance of O-antigen immunity in the host defense against shigellosis. |
| Databáze: | OpenAIRE |
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