A Phosphate Bound Universal Linker for DNA Synthesis
| Autor: | Ronald M. Cook, Daren J. Dick, Derek Hudson, Matthew H. Lyttle |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
DNA synthesis
DNA Phosphate Biochemistry Combinatorial chemistry Microspheres Uridine Phosphates chemistry.chemical_compound chemistry CpG site Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Phosphodiester bond Genetics Polystyrenes Organic chemistry Electrophoresis Polyacrylamide Gel Polystyrene Linker Chromatography High Pressure Liquid |
| Zdroj: | Nucleosides and Nucleotides. 18:1809-1824 |
| ISSN: | 0732-8311 |
| DOI: | 10.1080/07328319908044845 |
| Popis: | A uridine-based linker immobilized onto polystyrene beads at the 5′ terminus via a phosphodiester group and then used as a universal DNA synthesis support gives post synthesis DNA cleavage in 8 hrs or less without alkali metal salts. DNA produced with the new support was analyzed by HPLC, MALDI mass spectroscopy and PAGE. Each analysis showed DNA of equivalent quality to that produced with standard CPG supports, without contaminating materials resulting from linker or support backbone decomposition. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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