Diabetes Affects the Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP)-Like Immunoreactive Enteric Neurons in the Porcine Digestive Tract

Autor: Michał Bulc, Katarzyna Palus, Łukasz Zielonka, Marcin Nowicki, Jarosław Całka
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Blood Glucose
pig
medicine.medical_specialty
endocrine system
QH301-705.5
Swine
Population
Neuropeptide
Fluorescent Antibody Technique
Ileum
PACAP
Catalysis
Article
Descending colon
Diabetes Mellitus
Experimental
Inorganic Chemistry
Jejunum
enteric nervous system
Internal medicine
Diabetes Mellitus
Medicine
Animals
Physical and Theoretical Chemistry
Biology (General)
education
Molecular Biology
QD1-999
Spectroscopy
Neurons
Gastrointestinal tract
education.field_of_study
diabetes
business.industry
Stomach
Organic Chemistry
digestive
oral
and skin physiology
General Medicine
Computer Science Applications
Chemistry
medicine.anatomical_structure
Endocrinology
Organ Specificity
Pituitary Adenylate Cyclase-Activating Polypeptide
Enteric nervous system
gastrointestinal tract
business
Digestive System
Biomarkers
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 11
International Journal of Molecular Sciences, Vol 22, Iss 5727, p 5727 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms22115727
Popis: Diabetic gastroenteropathy is a common complication, which develops in patients with long-term diabetes. The pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide known for its cytoprotective properties and plays an important role in neuronal development, neuromodulation and neuroprotection. The present study was designed to elucidate, for the first time, the impact of prolonged hyperglycaemia conditions on a population of PACAP-like immunoreactive neurons in selected parts of the porcine gastrointestinal tract. The experiment was conducted on 10 juvenile female pigs assigned to two experimental groups: The DM group (pigs with streptozocin-induced diabetes) and the C group (control pigs). Diabetes conditions were induced by a single intravenous injection of streptozocin. Six weeks after the induction of diabetes, all animals were euthanised and further collected, and fixed fragments of the stomach, duodenum, jejunum, ileum and descending colon were processed using the routine double-labelling immunofluorescence technique. Streptozotocin-induced hyperglycaemia caused a significant increase in the population of PACAP-containing enteric neurons in the porcine stomach, small intestines and descending colon. The recorded changes may result from the direct toxic effect of hyperglycaemia on the ENS neurons, oxidative stress or inflammatory conditions accompanying hyperglycaemia and suggest that PACAP is involved in regulatory processes of the GIT function in the course of diabetes.
Databáze: OpenAIRE
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