Effect of exposition to chlorpyrifos upon plasmatic cholinesterases, hematology and blood biochemistry values in Bothrops asper (Serpentes: Viperidae)
| Autor: | Greivin Corrales, Guillermo León, Sonia M. Hernandez, Randall Arguedas, Marco D. Barquero, Aarón Gómez, Danilo Chacón |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Costa Rica
Male Insecticides medicine.medical_specialty Environmental Engineering 040301 veterinary sciences Bothrops asper Health Toxicology and Mutagenesis Physiology 010501 environmental sciences 01 natural sciences Group B 0403 veterinary science chemistry.chemical_compound Monocytosis Viperidae biology.animal Internal medicine medicine Animals Environmental Chemistry Bothrops Pesticides Butyrylcholinesterase 0105 earth and related environmental sciences Hematology biology Public Health Environmental and Occupational Health Albumin 04 agricultural and veterinary sciences General Medicine General Chemistry medicine.disease biology.organism_classification Pollution Organophosphates Acetylcholinesterase inhibitors chemistry Chlorpyrifos Female Biomarkers Blood Chemical Analysis |
| Zdroj: | Chemosphere, vol.205, pp.209-214 Kérwá Universidad de Costa Rica instacron:UCR |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2018.04.111 |
| Popis: | The terciopelo (Bothrops asper), is one of the most important venomous snakes in Costa Rica and common on agriculture where insecticides are frequently used for pest control. To assess the exposure to organophosphates on captive B. asper, an experiment using chlorpyrifos and butyrylcholinesterase (BChE), as a biomarker was conducted. In addition to BChE, hematology, aspartate aminotransferase (AST), total proteins (TP) and albumin were measured after exposure. Different concentrations of chlorpyrifos were used in Group A (0.1%) and B (1%), while the Control Group received distilled water; each group was composed of 5 snakes. Values of BChE, AST, TP, and albumin were measured before exposure, and at 6, 12, 24, 196, 360 and 528 h post-exposure. Hematology values were measured after 24 h post-exposure. As result, an important variation between subjects in all groups before exposure was obtained. Moreover, BChE activity showed 37% inhibition of Group A when compared to Control Group at 12 h post-exposure, and a higher inhibition of Group B (97%) related to Control Group, at 6 h postexposure. Recovery of BChE occurred towards 528 h, never reaching initial values. Despite some variation in the rest of parameters used, a marked relative lymphopenia and monocytosis occurred at 24 h, assuming stress as the main cause. Universidad de Costa Rica/[741-B7-106]/UCR/Costa Rica UCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiología UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biología UCR::Sedes Regionales::Sede del Caribe::Recinto Limón |
| Databáze: | OpenAIRE |
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