A vector expressing feline mature IL-18 fused to IL-1β antagonist protein signal sequence is an effective adjuvant to a DNA vaccine for feline leukaemia virus
| Autor: | David Argyle, Lesley Nicolson, D. Bain, Samantha Taylor, Elizabeth L. McMonagle, Linda Hanlon, David Onions, Lucy O'Donovan, Mathew C Golder, Oswald Jarrett, Michael McDonald, Angela M. Pacitti |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
FeLV
viruses Recombinant Fusion Proteins Genetic Vectors Retroviridae Proteins Oncogenic Biology Protein Sorting Signals Antibodies Viral Cat Diseases Virus Article DNA vaccination Plasmid Adjuvants Immunologic Bone Marrow hemic and lymphatic diseases Vaccines DNA Animals Vector (molecular biology) Gene Cytokine Expression vector General Veterinary General Immunology and Microbiology Leukemia Virus Feline Public Health Environmental and Occupational Health Interleukin-18 virus diseases Viral Vaccines Protein signal sequence Virology Interleukin-12 Tumor Virus Infections Infectious Diseases biology.protein Cats Molecular Medicine Antibody Vaccine Interleukin-1 Retroviridae Infections |
| Zdroj: | Vaccine |
| ISSN: | 1873-2518 0264-410X |
| Popis: | DNA vaccination using vectors expressing the gag/pol and env genes of feline leukaemia virus (FeLV) and plasmids encoding feline interleukin-12 (IL-12) and IL-18 completely protected cats from viraemia following challenge [Hanlon L, Argyle D, Bain D, Nicolson L, Dunham S, Golder MC, et al. Feline leukaemia virus DNA vaccine efficacy is enhanced by coadministration with interleukin-12 (IL-12) and IL-18 expression vectors. J Virol 2001;75:8424-33]. However, the relative contribution of each cytokine gene towards protection is unknown. This study aimed to resolve this issue. IL-12 and IL-18 constructs were modified to ensure effective expression, and bioactivity was demonstrated using specific assays. Kittens were immunised intramuscularly with FeLV DNA and various cytokine constructs. Together with control kittens, these were challenged oronasally with FeLV and monitored for 15 weeks. All six kittens given FeLV, IL-12 and IL-18 were protected from the establishment of persistent viraemia and four from latent infection. Of six kittens immunised with FeLV DNA and IL-18, all were protected from viraemia and five from latent infection. In contrast, three of five kittens given FeLV DNA and IL-12 became persistently viraemic. Therefore, the adjuvant effect on the FeLV DNA vaccine appears to reside in the expression of IL-18. |
| Databáze: | OpenAIRE |
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