Spectroscopic studies on the temperature-dependent molecular arrangements in hybrid chitosan/1,3-β-D-glucan polymeric matrices
Autor: | Grzegorz Kalisz, Izabela S. Pieta, Robert Nowakowski, Vladyslav Vivcharenko, Barbara Gieroba, Agnieszka Lewalska-Graczyk, Anna Sroka-Bartnicka, Agata Przekora, Paulina Kazimierczak |
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Rok vydání: | 2020 |
Předmět: |
beta-Glucans
Materials science Polymers 02 engineering and technology Microscopy Atomic Force Spectrum Analysis Raman Polysaccharide Biochemistry Chitosan 03 medical and health sciences chemistry.chemical_compound symbols.namesake X-ray photoelectron spectroscopy Structural Biology Spectroscopy Fourier Transform Infrared Molecule Fourier transform infrared spectroscopy Molecular Biology Protein secondary structure 030304 developmental biology chemistry.chemical_classification 0303 health sciences Molecular Structure Spectrum Analysis Temperature General Medicine 021001 nanoscience & nanotechnology Chemical engineering chemistry Attenuated total reflection symbols 0210 nano-technology Raman spectroscopy |
Zdroj: | International Journal of Biological Macromolecules. 159:911-921 |
ISSN: | 0141-8130 |
DOI: | 10.1016/j.ijbiomac.2020.05.155 |
Popis: | Chitosan/1,3-β-D-glucan matrices have been recently used in various biomedical applications. Within this study, the structural changes in hybrid polysaccharide chitosan/1,3-β-D-glucan matrices cross-linked at 70 °C and 80 °C were detected with Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR FT-IR) and Raman spectroscopy enabled thorough insights into molecular structure of studied biomaterials, whereas X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) provided their surface characteristics with confirmation of their effective and non-destructive properties. There are temperature-dependent differences in the chemical interactions between 1,3-β-D-glucan units and N-glucosamine in chitosan, resulting in surface polarity changes. The second order derivatives and deconvolution revealed the alterations in the secondary structure of studied matrices, along with different sized grain-like structures revealed by AFM. Since surface physicochemical properties of biomaterials have great impact on cell behavior, abovementioned techniques may allow to optimize and modify the preparation of polymeric matrices with desired features. |
Databáze: | OpenAIRE |
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