High‐fluence light emitting diode‐red light inhibits cell cycle progression in human dermal fibroblasts
| Autor: | Natasha Masub, Evan Austin, Jared Jagdeo, Alisen Huang |
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| Rok vydání: | 2020 |
| Předmět: |
Light
Cell cycle progression Regulator General Physics and Astronomy 01 natural sciences Fluence Article General Biochemistry Genetics and Molecular Biology 010309 optics Dermal fibroblast Cellular mechanism Fibrosis 0103 physical sciences medicine Humans General Materials Science Red light Fibroblast Cell Proliferation Skin Chemistry 010401 analytical chemistry General Engineering General Chemistry Fibroblasts medicine.disease 0104 chemical sciences medicine.anatomical_structure Cancer research Cell Division |
| Zdroj: | J Biophotonics |
| ISSN: | 1864-0648 1864-063X |
| Popis: | Skin fibrosis is a debilitating feature of several systemic and dermatologic diseases. While current treatment options carry significant risk of side effects and recurrence, high-fluence light emitting diode-generated red light (LED-RL) is an alternative therapeutic that is safe, non-invasive, and accessible. We previously demonstrated LED-RL decreases fibroblast proliferation, a key pathogenic component of fibrosis. However, the cellular mechanism by which high fluence LED-RL modulates fibroblast proliferation is unclear. Herein, we explored the effects of high fluence LED-RL on human dermal fibroblast cell cycle progression. We demonstrate that LED-RL at 640 J/cm(2) induced significant arrest of cells in G(0)/G(1) compared to temperature-matched control. This was accompanied by a corresponding increase in expression of checkpoint regulator p53 in irradiated cells. These data demonstrate high fluence LED-RL may exert its anti-proliferative effect on fibroblasts by inducing G(0)/G(1) arrest. Further, this study provides insight into the molecular mechanism underlying LED-RL as an anti-fibrotic therapeutic. |
| Databáze: | OpenAIRE |
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