Development and validation of a lateral flow assay (LFA) for the determination of IgG-antibodies to Pr3 (cANCA) and MPO (pANCA)
| Autor: | K Conrad, N Offermann, Marvin J. Fritzler, M Fooke Achterrath |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Myeloblastin Immunology Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis Enzyme-Linked Immunosorbent Assay Antibodies Antineutrophil Cytoplasmic Proteinase 3 Predictive Value of Tests Immunology and Allergy Medicine Humans Fluorescent Antibody Technique Indirect Point of care Peroxidase Immunoassay biology business.industry Panca Autoantibody Reproducibility of Results Serum samples medicine.disease biology.organism_classification Myeloperoxidase Case-Control Studies Immunoglobulin G Luminescent Measurements biology.protein Antibody business Granulomatosis with polyangiitis Biomarkers |
| Zdroj: | Journal of immunological methods. 403(1-2) |
| ISSN: | 1872-7905 |
| Popis: | The timely diagnosis of vasculopathies, such as granulomatosis with polyangiitis, has important implications for the favorable clinical outcome of these diseases. In the clinical setting, autoantibodies to proteinase 3 (Pr3) and myeloperoxidase (MPO) have been shown to be valuable adjuncts to an early and accurate diagnosis. The sensitive and specific detection of anti-Pr3 and anti-MPO was shown using a point of care device that employed rapid Lateral Flow Technologies. The validation of the lateral flow assay (LFA) was performed with serum samples collected in two Reference Laboratories and showed excellent results that were comparable to widely accepted and used ELISA. The advantage of the LFA is the flexibility to be used as an economical, point of care diagnostic device, features that are especially important for an early and accurate diagnosis and the prompt initiation of appropriate treatment so as to avoid inevitable development of undue complications of these diseases such as disseminated organ involvement, e.g. renal failure. |
| Databáze: | OpenAIRE |
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