Intraflagellar transport proteins cycle between the flagellum and its base
| Autor: | Johanna Buisson, Thibault Lagache, Jean-Christophe Olivo-Marin, Thierry Blisnick, Philippe Bastin, Nicolas Chenouard |
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| Přispěvatelé: | Biologie Cellulaire des Trypanosomes, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Analyse d'Images Quantitative (AIQ), Work in the Trypanosome Cell Biology Unit is funded by the Institut Pasteur, the Centre National de la Recherche Scientifique (CNRS), and by two grants of the Agence Nationale de la recherche (ANR-08-MIE-027, ANR-09-GENOPAT-R09088KS). J.B. was funded by fellowships from the Ministere de l'Enseignement Supérieur et de la Recherche (ED387), the Fondation pour la Recherche Médicale and Pasteur-Weizmann. N.C. was funded by a doctoral fellowship from C'Nano Ile-de-France. T.L. is funded by a Pasteur Transversal Research Program (PTR387)., ANR-08-MIEN-0027,SENSOTRYPA,Rôles sensoriels du flagelle au cours du cycle parasitaire du trypanosome africain(2008), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2013 |
| Předmět: |
MESH: Bayes Theorem
Trypanosoma brucei brucei Dynein Protozoan Proteins Base (geometry) MESH: Carrier Proteins Trypanosoma brucei Flagellum Trypanosome MESH: Flagella Cilia and flagella 03 medical and health sciences 0302 clinical medicine MESH: Cilia Microtubule Intraflagellar transport [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology Cilia MESH: Protozoan Proteins 030304 developmental biology 0303 health sciences biology Cilium MESH: Trypanosoma brucei brucei Fluorescence recovery after photobleaching Bayes Theorem Cell Biology Anatomy biology.organism_classification Flagella Biophysics Carrier Proteins MESH: Fluorescence Recovery After Photobleaching 030217 neurology & neurosurgery Fluorescence Recovery After Photobleaching |
| Zdroj: | Journal of Cell Science Journal of Cell Science, 2013, 126 (1), pp.327-338. ⟨10.1242/jcs.117069⟩ Journal of Cell Science, Company of Biologists, 2013, 126 (1), pp.327-338. ⟨10.1242/jcs.117069⟩ |
| ISSN: | 1477-9137 0021-9533 |
| Popis: | Summary Intraflagellar transport (IFT) is necessary for the construction of cilia and flagella. IFT proteins are concentrated at the base of the flagellum but little is known about the actual role of this pool of proteins. Here, IFT was investigated in Trypanosoma brucei, an attractive model for flagellum studies, using GFP fusions with IFT52 or the IFT dynein heavy chain DHC2.1. Tracking analysis by a curvelet method allowing automated separation of forward and return transport demonstrated a uniform speed for retrograde IFT (5 µm s−1) but two distinct populations for anterograde movement that are sensitive to temperature. When they reach the distal tip, anterograde trains are split into three and converted to retrograde trains. When a fast anterograde train catches up with a slow one, it is almost twice as likely to fuse with it rather than to overtake it, implying that these trains travel on a restricted set of microtubules. Using photobleaching experiments, we show for the first time that IFT proteins coming back from the flagellum are mixed with those present at the flagellum base and can reiterate a full IFT cycle in the flagellum. This recycling is dependent on flagellum length and IFT velocities. Mathematical modelling integrating all parameters actually reveals the existence of two pools of IFT proteins at the flagellum base, but only one is actively engaged in IFT. |
| Databáze: | OpenAIRE |
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