A canine adenovirus type 2 vaccine vector confers protection against foot-and-mouth disease in guinea pigs

Autor: Charly Pignon, Labib Bakkali-Kassimi, Bernard Klonjkowski, David J. Lefebvre, Annebel De Vleeschauwer, Annabelle Garnier, Sandrine A. Lacour, Kris De Clercq, Stéphan Zientara, Fleur Watier, Xiaocui Zhou
Přispěvatelé: Sciensano [Bruxelles], Réseau International des Instituts Pasteur (RIIP), Institut National de la Recherche Agronomique (INRA), China Animal Health and Epidemiology Center, Virologie UMR1161 (VIRO), Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA), École nationale vétérinaire d'Alfort (ENVA), Belgian Federal Public Service for Health, Food Chain Safety and Environment [RI14/2-26], French Agence Nationale de la Recherche [ANR-14-ANWA-0007-01], European Project: 226556,EC:FP7:KBBE,FP7-KBBE-2008-2B,FMD-DISCONVAC(2009), De Vleeschauwer, Annebel R., Zhou, Xiaocui, Klonjkowski, Bernard, École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), École nationale vétérinaire - Alfort (ENVA)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Vaccine
Vaccine, Elsevier, 2018, 36 (16), pp.2193-2198. ⟨10.1016/j.vaccine.2018.02.074⟩
Vaccine 16 (36), 2193-2198. (2018)
Vaccine, 2018, 36 (16), pp.2193-2198. ⟨10.1016/j.vaccine.2018.02.074⟩
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2018.02.074⟩
Popis: International audience; Vaccination is a key element in the control of foot-and-mouth disease (FMD). The majority of the antigenic sites that induce protective immune responses are localized on the FMD virus (FMDV) capsid that is formed by four virus-encoded structural proteins, VP1 to VP4. In the present study, recombinant canine adenovirus type 2 (CAV2)-based FMD vaccines, Cav-Pl/3C R degrees and Cav-VP1 R degrees, respectively expressing the structural P1 precursor protein along with the non-structural 3C protein or expressing the structural VP1 protein of the FMDV strain O/FRA/1/2001, were evaluated as novel vaccines against FMD. A strong humoral immune response was elicited in guinea pigs (GP) following immunization with Cav-P1/3C R, while administration of Cav-VP1 R degrees did not induce a satisfying antibody response in GP or mice. GP were then used as an experimental model for the determination of the protection afforded by the Cav/P1 R degrees vaccine against challenge with the FMDV strain O-1 Manisa/Turkey/1969. The Cav-P1/3C R vaccine protected GP from generalized FMD to a similar extent as a high potency double-oil emulsion O-1 Manisa vaccine. The results of the present study show that CAV2-based vector vaccines can express immunogenic FMDV antigens and offer protection against generalized FMD in GP. This suggest that Cav-P1 R FMDV vaccine may protect natural host species from FMD. In combination with an appropriate diagnostic test, the Cav-P1 R degrees FMDV vaccine may also serve as a marker vaccine to differentiate vaccinated from infected animals.
Databáze: OpenAIRE
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