Anaerobic Dechlorination by a Humin-Dependent Pentachlorophenol-Dechlorinating Consortium under Autotrophic Conditions Induced by Homoacetogenesis
| Autor: | Takuya Kasai, Takanori Awata, Mahasweta Laskar, Arata Katayama |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
CO2
Pentachlorophenol Halogenation Health Toxicology and Mutagenesis lcsh:Medicine Electrons Dehalobacter 010501 environmental sciences 01 natural sciences reductive dechlorination Article homoacetogenesis 03 medical and health sciences chemistry.chemical_compound Bioremediation Syntrophy Reductive dechlorination Anaerobiosis Autotroph Groundwater Humic Substances 030304 developmental biology 0105 earth and related environmental sciences Clostridium Autotrophic Processes 0303 health sciences H2 humin biology Chemistry Microbiota lcsh:R Public Health Environmental and Occupational Health biology.organism_classification Anoxic waters acetogens Biodegradation Environmental autotrophic Peptococcaceae Environmental chemistry Humin Oxidation-Reduction Water Pollutants Chemical |
| Zdroj: | International Journal of Environmental Research and Public Health Volume 16 Issue 16 International Journal of Environmental Research and Public Health, Vol 16, Iss 16, p 2873 (2019) |
| ISSN: | 1660-4601 |
| DOI: | 10.3390/ijerph16162873 |
| Popis: | Anoxic aquifers suffer from energy limitations due to the unavailability of organic substrates, as dictated by hydrogen (H2) for various electron-accepting processes. This deficiency often results in the accumulation of persistent organic pollutants, where bioremediation using organic compounds often leads to secondary contamination. This study involves the reductive dechlorination of pentachlorophenol (PCP) by dechlorinators that do not use H2 directly, but rather through a reduced state of humin&mdash a solid-phase humic substance&mdash as the extracellular electron donor, which requires an organic donor such as formate, lactate, etc. This shortcoming was addressed by the development of an anaerobic mixed culture that was capable of reductively dechlorinating PCP using humin under autotrophic conditions induced by homoacetogenesis. Here, H2 was used for carbon-dioxide fixation to acetate the acetate produced was used for the reduction of humin and consequently used for dechlorination through reduced humin. The 16SrRNA gene sequencing analysis showed Dehalobacter and Dehalobacterium as the possible dechlorinators, while Clostridium and Oxobacter were identified as the homoacetogens. Thus, this work contributes to the development of an anaerobic consortium that balanced H2 dependency, where efficiency of humin reduction extends the applicability of anaerobic microbial remediation in aquifers through autotrophy, syntrophy, and reductive dechlorination. |
| Databáze: | OpenAIRE |
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