Cell targeting strategy affects the intracellular trafficking of liposomes altering loaded doxorubicin release kinetics and efficacy in endothelial cells
| Autor: | Anthoula Arta, Michael Larsen, Paul J. Kempen, Thomas Lars Andresen, Anne Zebitz Eriksen, Jannik B. Larsen, Andrew J. Urquhart |
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| Rok vydání: | 2020 |
| Předmět: |
Endosome
Cell Survival Drug Compounding Pharmaceutical Science 02 engineering and technology Endosomes 030226 pharmacology & pharmacy 03 medical and health sciences 0302 clinical medicine In vivo medicine Humans Doxorubicin Cells Cultured Cell Proliferation Liposome Dose-Response Relationship Drug Chemistry Endothelial Cells 021001 nanoscience & nanotechnology Lipids Endocytosis Cell biology Endothelial stem cell Drug Liberation Kinetics Drug delivery Liposomes Nanocarriers 0210 nano-technology Lysosomes Intracellular medicine.drug |
| Zdroj: | International journal of pharmaceutics. 588 |
| ISSN: | 1873-3476 |
| Popis: | Targeting nanocarrier drug delivery systems, that deliver drug payloads to the site of disease action, are frequently viewed as the future of nanocarrier based therapies but have struggled to breakthrough to the clinic in comparison to non-targeting counterparts. Using unilamellar liposomes as model nanocarriers, we show that cell targeting strategy (electrostatic, ligand and antigen) influences both the intracellular fate of the liposomes and the corresponding efficacy of the loaded drug, doxorubicin, in endothelial cells. We show that increased liposome uptake by cells does not translate to improved efficacy in this scenario but that liposome intracellular trafficking, particularly distribution between recycling endosomes and lysosomes, influences in vitro efficacy. Choosing targeting strategies that promote desired nanocarrier intracellular trafficking may be a viable strategy to enhance the in vivo efficacy of drug delivery systems. |
| Databáze: | OpenAIRE |
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