Exosomal microRNAs as tumor markers in epithelial ovarian cancer

Autor: Klaus Pantel, Volkmar Müller, Leticia Oliveira-Ferrer, Ines Stevic, Chi Pan, Qingtao Ni, Heidi Schwarzenbach
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Adult
epithelial ovarian cancer
Cancer Research
endocrine system diseases
Cystadenoma
exosomes
Carcinoma
Ovarian Epithelial
lcsh:RC254-282
Disease-Free Survival
Pathogenesis
Ovarian Cystadenoma
03 medical and health sciences
0302 clinical medicine
microRNA
Genetics
medicine
Humans
Circulating MicroRNA
RNA
Neoplasm
Research Articles
ovarian cystadenoma
Tumor marker
Aged
Aged
80 and over
Ovarian Neoplasms
Cell growth
business.industry
apoptosis
General Medicine
Middle Aged
medicine.disease
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Microvesicles
female genital diseases and pregnancy complications
Survival Rate
030104 developmental biology
cell proliferation
Oncology
Apoptosis
030220 oncology & carcinogenesis
Cancer research
Molecular Medicine
Female
prognosis
Ovarian cancer
business
Research Article
Zdroj: Molecular Oncology, Vol 12, Iss 11, Pp 1935-1948 (2018)
Molecular Oncology
ISSN: 1574-7891
1878-0261
Popis: Specific microRNAs (miRNAs) are packaged in exosomes that regulate processes in tumor development and progression. The current study focuses on the influence of exosomal miRNAs in the pathogenesis of epithelial ovarian cancer (EOC). MiRNA profiles were determined in exosomes from plasma of 106 EOC patients, eight ovarian cystadenoma patients, and 29 healthy women by TaqMan real-time PCR-based miRNA array cards containing 48 different miRNAs. In cell culture experiments, the impact of miR-200b and miR-320 was determined on proliferation and apoptosis of ovarian cancer cell lines. We report that miR-21 (P = 0.0001), miR-100 (P = 0.034), miR-200b (P = 0.008), and miR-320 (P = 0.034) are significantly enriched, whereas miR-16 (P = 0.009), miR-93 (P = 0.014), miR-126 (P = 0.012), and miR-223 (P = 0.029) are underrepresented in exosomes from plasma of EOC patients as compared to those of healthy women. The levels of exosomal miR-23a (P = 0.009, 0.008) and miR-92a (P = 009, 0.034) were lower in ovarian cystadenoma patients than in EOC patients and healthy women, respectively. The exosomal levels of miR-200b correlated with the tumor marker CA125 (P = 0.002) and patient overall survival (P = 0.019). MiR-200b influenced cell proliferation (P = 0.0001) and apoptosis (P < 0.008). Our findings reveal specific exosomal miRNA patterns in EOC and ovarian cystadenoma patients, which are indicative of a role of these miRNAs in the pathogenesis of EOC.
Databáze: OpenAIRE
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