Erythrocyte calcium metabolism. Calcium exchange in normal and sickle-cell-anaemia erythrocytes
| Autor: | P E Smariga, B F Cameron |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Erythrocytes
Sodium medicine.medical_treatment Cell Erythrocytes Abnormal Salt (chemistry) chemistry.chemical_element Anemia Sickle Cell In Vitro Techniques Calcium Biochemistry Phosphates Cell membrane chemistry.chemical_compound medicine Humans Molecular Biology Saline chemistry.chemical_classification Calcium metabolism Cell Membrane Cell Biology Phosphate Kinetics medicine.anatomical_structure chemistry Research Article |
| Zdroj: | Biochemical Journal. 156:577-583 |
| ISSN: | 0264-6021 |
| Popis: | Under exchange conditions (no net increase in calcium), erythrocytes incubated in isoosmotic phosphate-buffered saline have an exchangeable calcium pool comprising about 10% of the total erythrocyte calcium. This pool reaches exchange equilibrium, for either inward-directed or outward-directed transfer of the 45Ca-exchange label, with a half-time of about 20 min. The uptake of Ca2+ requires phosphate, even under hypo-osmotic conditions, where the calcium loading expected as the cells swell is obtained only when phosphate is present. The phosphate requirement is not due to Ca2+ transport as a phosphate salt. This exchangeable-calcium pool is also present in sickle-cell-anemia erythrocytes, and comprises a similar proportion of total cellular calcium. |
| Databáze: | OpenAIRE |
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