MALDI-2 on a Trapped Ion Mobility Quadrupole Time-of-Flight Instrument for Rapid Mass Spectrometry Imaging and Ion Mobility Separation of Complex Lipid Profiles
| Autor: | Klaus Dreisewerd, Jens Höhndorf, Bram Heijs, Simeon Vens-Cappell, Annika Koch, Jens Soltwisch |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Ion-mobility spectrometry Analytical chemistry 010402 general chemistry Mass spectrometry 01 natural sciences Mass spectrometry imaging Analytical Chemistry Ion law.invention law Ionization Ion Mobility Spectrometry Testis Animals chemistry.chemical_classification Chemistry Biomolecule 010401 analytical chemistry Brain Lipidome Laser Lipids Rats 0104 chemical sciences Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization |
| Zdroj: | Analytical Chemistry, 92(13), 8697-8703. AMER CHEMICAL SOC |
| Popis: | Matrix-assisted laser desorption/ionization combined with laser-induced postionization (MALDI-2) is a recently introduced method for enhanced mass spectrometry imaging of numerous classes of biomolecules, including phospho- and glycolipids in tissue sections at high lateral resolution. Here we describe the first adaptation of the technology to a Bruker timsTOF fleX mass spectrometer. Upon use of a 1 kHz postionization laser, MALDI-2 produces a sizable increase in the number of detected features as well as in ion signal intensities. This enhancement is similar to that described previously for low repetition rate MALDI-2 systems, but now enables substantially enhanced measurement speeds. In our proof-of-concept study, we furthermore demonstrate, on examples of rat brain and testis tissue sections, that the combination of MALDI-2 with the trapped ion mobility spectrometry (TIMS) functionality of the instrument can crucially support unravelling the complex molecular composition of the lipidome. Numerous isomeric/isobaric ion species are successfully separated upon using the collisional cross section (CCS) as additional specific physical property. With the possibilities of high data acquisition speed or high separation powers in combination with the increased sensitivity of MALDI-2 available in one instrument, the described methodology could be a valuable tool in many areas of biological and medical research. |
| Databáze: | OpenAIRE |
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