Cassette recruitment in the chromosomal Integron ofVibrio cholerae
| Autor: | Claire Vit, Xavier Eyer, Vincent Parissi, Florian Fournes, Egill Richard, Didier Mazel, Clemence Whiteway, Céline Loot, Delphine Lapaillerie |
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| Přispěvatelé: | Plasticité du Génome Bactérien - Bacterial Genome Plasticity (PGB), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), École Doctorale Bio Sorbonne Paris Cité [Paris] (ED BioSPC), Université Sorbonne Paris Cité (USPC)-Université de Paris (UP), Institut de Cancérologie Strasbourg (ICANS), Vrije Universiteit Brussel (VUB), Hôpital Lariboisière-Fernand-Widal [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Microbiologie cellulaire et moléculaire et pathogénicité (MCMP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), This work was supported by the French Government’s Investissement d’Avenir program Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ [ANR-10-LABX-62- IBEID] and ‘Fondation pour la Recherche Médicale’, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Faculty of Sciences and Bioengineering Sciences, Department of Bio-engineering Sciences, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), École Doctorale Bio Sorbonne Paris Cité [Paris] (ED562 - BioSPC), Université Sorbonne Paris Cité (USPC)-Université Paris Cité (UPCité), Microbiologie Fondamentale et Pathogénicité (MFP), Institut Pasteur, Centre National de la Recherche Scientifique, the French Government’s Investissement d’Avenir program Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases [ANR-10-LABX-62- IBEID], Fondation pour la Recherche Médicale and Ecole Doctorale Complexité du Vivant. Funding for open access charge: Institut Pasteur, We would like to thank Evelyne Krin for providing the N16961 recA mutant and Sandra Arroyo-Beck and Marcos Manero for their experimental help. We also thank Jason Bland for helpful reading of the manuscript and all the lab members for helpful discussion., Author contributions: C.L., D.M. and V.P. designed the research. C.L., C.V., E.R., F.F., C.W., X.E. and D.L. performed the experiments. C.L. and C.V. wrote the draft of the manuscript. All authors read, amended the manuscript, and approved its final version. |
| Rok vydání: | 2020 |
| Předmět: |
MESH: Integrases
AcademicSubjects/SCI00010 [SDV]Life Sciences [q-bio] Genome Integrity Repair and Replication Integron medicine.disease_cause Integrons/genetics Integrons Plasmid SOS response Vibrio cholerae Recombination Genetic Genetics MESH: Gene Expression Regulation Bacterial 0303 health sciences Rec A Recombinases/genetics biology MESH: Escherichia coli [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] Recombination Genetic/genetics MESH: Integrons Integrase MESH: Rec A Recombinases Adaptive selection MESH: Recombination Genetic Gene Transfer Horizontal Integrases/genetics Chromosomes 03 medical and health sciences Vibrio cholerae/genetics Escherichia coli medicine 030304 developmental biology Integrases 030306 microbiology Chromosomes/genetics Gene Transfer Horizontal/genetics Gene Expression Regulation Bacterial Gene Expression Regulation Bacterial/genetics biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Gene Transfer Horizontal Rec A Recombinases Transformation (genetics) Escherichia coli/metabolism biology.protein bacteria MESH: Chromosomes MESH: Vibrio cholerae Bacteria |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, 2021, 49 (10), pp.5654-5670. ⟨10.1093/nar/gkab412⟩ |
| ISSN: | 0305-1048 1362-4962 |
| DOI: | 10.1101/2020.11.24.395434 |
| Popis: | Integrons are genetic systems conferring to bacteria a rapid adaptation capability. The integron integrase is able to capture, stockpile and shuffle novel functions embedded in cassettes. This involves the recognition of both substrates, theattIsite, and the cassette associatedattCsites. Integrons can be sedentary and chromosomally located (SCI) or, carried by conjugative plasmids (Mobile Integron, MI), hence favoring their dissemination among bacteria. Here, for the first time, we investigate the cassette recruitment in theVibrio choleraeSCI during conjugation and natural transformation. We demonstrated that horizontally transferred cassette can be recruited inside the chromosomal integron. The endogenous integrase expression is sufficiently triggered, after SOS response induction mediated by the entry of single-stranded cassettes during conjugation and natural transformation, to mediate significant cassette insertion. We demonstrate that theattIAinsertion is preferential, despite the presence of 180attCsites in the integron array. Thanks to the presence of a promoter in theattIAsite vicinity, all these newly inserted cassettes are expressed and prone to adaptive selection. We also show that the RecA protein is critical for cassette recruitment inV. choleraeSCI but not in MIs. Moreover,a contrarioto MIs, theV. choleraeSCI is not active in others bacterial hosts. MIs might have evolved from the SCIs by overcoming host factors, which would explain their large dissemination in bacteria and their role in the antibioresistance expansion. |
| Databáze: | OpenAIRE |
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