Human nerve growth factor beta (hNGF-beta): mammary gland specific expression and production in transgenic rabbits
| Autor: | Gottfried Brem, Urban Besenfelder, Mathias Müller, Michaela Fleischmann, M Tögel, N Zinovieva, I Bartke, A Grossmann, S. Coulibaly, M Wozny |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Transgenic rabbit Transgene Biophysics Chick Embryo Biology Biochemistry PC12 Cells Mass Spectrometry law.invention Animals Genetically Modified Mammary Glands Animal Dorsal root ganglion Western blot Structural Biology law Complementary DNA Ganglia Spinal Human nerve growth factor beta PC12W2 cell Genetics medicine Neurites Animals Humans Lactation Nerve Growth Factors RNA Messenger Molecular Biology Cells Cultured Cell Size Messenger RNA medicine.diagnostic_test Dose-Response Relationship Drug Bovine αs1-casein Biological activity Cell Biology Molecular biology Recombinant Proteins Rats Dorsal root ganglion neuron Nerve growth factor medicine.anatomical_structure Milk Organ Specificity Recombinant DNA Female Rabbits |
| Zdroj: | FEBS letters. 444(1) |
| ISSN: | 0014-5793 |
| Popis: | Transgenic rabbits carrying gene constructs encoding human nerve growth factor beta (hNGF-beta) cDNA were generated. Expression of hNGF-beta mRNA was restricted to the mammary gland of lactating rabbits. Western Blot analysis revealed a polypeptide of 13.2 kDa in the milk of transgenic animals. hNGF-beta was purified from the milk by a two-step chromatographic procedure. Electrospray mass spectroscopy analysis of purified hNGF-beta depicted a molecular weight of 13,261 Da per subunit. The biological activity of the hNGF-beta was tested using PC12W2 cells and cultures of dorsal root ganglion neurons from chicken embryos. Crude defatted milk from transgenic animals and purified hNGF-beta demonstrated full biological activity when compared to commercial recombinant hNGF-beta. |
| Databáze: | OpenAIRE |
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