| Autor: |
Filice, Dominic, Dhahri, Wahiba, Joell L. Solan, Lampe, Paul D., Steele, Erin, Milani, Nikita, Biber, Benjamin Van, Zhu, Wei-Zhong, Valdman, Tamilla Sadikov, Romagnolo, Rocco, Otero-Cruz, José David, Hauch, Kip D., Kay, Matthew W., Sarvazyan, Narine, Laflamme, Michael A. |
| Rok vydání: |
2020 |
| DOI: |
10.6084/m9.figshare.13018086.v2 |
| Popis: |
Additional file 4: Supplementary Fig. S4. Simultaneous di-2-ANEPEQ imaging and direct electrode recording of hESC-CM graft electrical activity. A cryoinjured heart with hESC-CM graft (H7 line) was impaled with a sharp electrode within the graft tissue to correlate the di-2-ANEPEQ-derived voltage signal with direct intracellular voltage recordings. A: Epicardial still image taken on the di-2-ANEPEQ channel, showing the uncoupled hESC-CM graft footprint (yellow dotted line) inside the cryoinjury region (white dotted line) and the recording electrode positioned in graft tissue. Two ROIs are indicated: one in host myocardium outside the cryoinjury zone and one in hESC-CM graft tissue overlying the tip of the recording electrode. B: Simultaneously acquired oAPs for these two ROIs, as well as the simultaneously acquired direct voltage and ECG recordings. Note the excellent temporal correlation between the direct electrode recordings and di-2-ANEPEQ-derived oAPs from this hESC-CM graft, which was uncoupled from the host. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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