Effect of Remote Ischemic Preconditioning on Systemic Toxicity and Ototoxicity Induced by Cisplatin in Rats: Role of TNF-α and Nitric Oxide
| Autor: | Batista Ama, de Freitas Mr, Brito Gac, Brito Ynf, Soares Pmg, Martins Cds, Ribeiro Ra, Martins Mjb |
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| Rok vydání: | 2017 |
| Předmět: |
Male
medicine.medical_treatment Ischemia Nitric Oxide Synthase Type II Antineoplastic Agents Pharmacology Nitric Oxide Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Hearing Ototoxicity Evoked Potentials Auditory Brain Stem medicine Animals Rats Wistar Ischemic Preconditioning Saline Cisplatin biology Tumor Necrosis Factor-alpha business.industry medicine.disease Immunohistochemistry Cochlea Nitric oxide synthase Otorhinolaryngology chemistry 030220 oncology & carcinogenesis biology.protein Ischemic preconditioning Tumor necrosis factor alpha business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | ORL. 79:336-346 |
| ISSN: | 1423-0275 0301-1569 |
| DOI: | 10.1159/000485514 |
| Popis: | Background/Aims: Cisplatin is a chemotherapeutic agent. The use of remote ischemic preconditioning (RIPC) was proposed after the observation that ischemic preconditioning of a cardiac vascular area could protect another completely distinctly. Methods: This is an experimental study. Male Wistar rats were anesthetized, and they underwent a hearing evaluation via measurement of the brainstem auditory evoked potential (BSAEP). Then, cisplatin was administered intraperitoneally (IP) at a dose of 8 mg/kg/day for 4 consecutive days to group 1, whereas saline solution was administered IP to group 2. In groups 3 and 4, ischemia of the right hind paw was performed for 10 min, followed by reperfusion for 30 min, after which cisplatin or saline was administered IP to group 3 or group 4, respectively. Afterwards, all animals were evaluated via the BSAEP. The right cochlea was dissected for immunohistochemistry. Results: RIPC lowered the increase in BSAEP of the animals treated with cisplatin (p = 0.0146). Weight loss decreased in the animals subjected to RIPC (p < 0.005). In group 3, RIPC reversed immunostaining for tumor necrosis factor-α and inducible nitric oxide synthase in the stria vascularis injured by cisplatin (p < 0.05). Conclusion: RIPC protects against systemic toxicity and ototoxicity induced by cisplatin in rats. |
| Databáze: | OpenAIRE |
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