Immunomodulation by interleukin-33 is protective in stroke through modulation of inflammation
| Autor: | Iurii Kidin, Joan Montaner, Jari Koistinaho, Sanna Loppi, Paula Korhonen, Teresa García-Berrocoso, Dolors Giralt, Katja A. Puttonen, Sara Wojciechowski, Hiramani Dhungana, Šárka Lehtonen, Sighild Lemarchant, Tarja Malm, Katja M. Kanninen, Minna Oksanen, Eveliina Pollari |
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| Rok vydání: | 2015 |
| Předmět: |
Male
T-Lymphocytes medicine.medical_treatment Immunology Inflammation Motor Activity Pharmacology Brain Ischemia Mice Behavioral Neuroscience Immune system medicine Animals Humans Receptors Somatostatin Receptor Stroke Cells Cultured Aged Mice Inbred BALB C Microglia Endocrine and Autonomic Systems business.industry Macrophages Brain Interleukin Interleukin-33 medicine.disease Recombinant Proteins Interleukin 33 Cytokine medicine.anatomical_structure Astrocytes Cytokines Female Interleukin-4 medicine.symptom business Spleen |
| Zdroj: | Brain, Behavior, and Immunity. 49:322-336 |
| ISSN: | 0889-1591 |
| DOI: | 10.1016/j.bbi.2015.06.013 |
| Popis: | Cerebral stroke induces massive Th1-shifted inflammation both in the brain and the periphery, contributing to the outcome of stroke. A Th1-type response is neurotoxic whereas a Th2-type response is accompanied by secretion of anti-inflammatory cytokines, such as interleukin-4 (IL-4). Interleukin-33 (IL-33) is a cytokine known to induce a shift towards the Th2-type immune response, polarize macrophages/microglia towards the M2-type, and induce production of anti-inflammatory cytokines. We found that the plasma levels of the inhibitory IL-33 receptor, sST2, are increased in human stroke and correlate with a worsened stroke outcome, suggesting an insufficient IL-33-driven Th2-type response. In mouse, peripheral administration of IL-33 reduced stroke-induced cell death and improved the sensitivity of the contralateral front paw at 5days post injury. The IL-33-treated mice had increased levels of IL-4 in the spleen and in the peri-ischemic area of the cortex. Neutralization of IL-4 by administration of an IL-4 antibody partially prevented the IL-33-mediated protection. IL-33 treatment also reduced astrocytic activation in the peri-ischemic area and increased the number of Arginase-1 immunopositive microglia/macrophages at the lesion site. In human T-cells, IL-33 treatment induced IL-4 secretion, and the conditioned media from IL-33-exposed T-cells reduced astrocytic activation. This study demonstrates that IL-33 is protective against ischemic insult by induction of IL-4 secretion and may represent a novel therapeutic approach for the treatment of stroke. |
| Databáze: | OpenAIRE |
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