Squalene epoxidase, located on chromosome 8q24.1, is upregulated in 8q+ breast cancer and indicates poor clinical outcome in stage I and II disease
| Autor: | Heike Pospisil, C. M. Schlotter, D. Kemming, Eberhard Korsching, Alice Wang, U. Vogt, Mike W. Helms, Cornelia Liedtke, Horst Buerger, Burkhard Brandt, S Y Chan |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cancer Research
medicine.medical_specialty DNA Complementary Squalene monooxygenase 8q Estrogen receptor Breast Neoplasms LIV-1 Biology breast cancer Breast cancer Internal medicine medicine Humans RNA Messenger Neoplasm Metastasis skin and connective tissue diseases Molecular Diagnostics Survival analysis DNA Primers Oligonucleotide Array Sequence Analysis Base Sequence Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling 7p Chromosome Mapping Cancer Prognosis medicine.disease Survival Analysis oestrogen receptor Gene expression profiling Treatment Outcome Endocrinology Squalene Monooxygenase Oncology Cancer research squalene epoxidase Breast disease Tamoxifen Chromosomes Human Pair 8 medicine.drug |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/sj.bjc.6604556 |
| Popis: | Gains of chromosomes 7p and 8q are associated with poor prognosis among oestrogen receptor-positive (ER+) stage I/II breast cancer. To identify transcriptional changes associated with this breast cancer subtype, we applied suppression subtractive hybridisation method to analyse differentially expressed genes among six breast tumours with and without chromosomal 7p and 8q gains. Identified mRNAs were validated by real-time RT–PCR in tissue samples obtained from 186 patients with stage I/II breast cancer. Advanced statistical methods were applied to identify associations of mRNA expression with distant metastasis-free survival (DMFS). mRNA expression of the key enzyme of cholesterol biosynthesis, squalene epoxidase (SQLE, chromosomal location 8q24.1), was associated with ER+ 7p+/8q+ breast cancer. Distant metastasis-free survival in stage I/II breast cancer cases was significantly inversely related to SQLE mRNA in multivariate Cox analysis (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|