Hormonal Cross-Talk Between Auxin and Ethylene Differentially Regulates the Expression of Two Members of the 1-Aminocyclopropane-1-Carboxylate Oxidase Gene Family in Rice (Oryza sativa L.)
| Autor: | Mi Young Park, Yong Gu Cho, Moo Young Eun, Woo Taek Kim, Hyun Sook Chae, Bin G. Kang, Myung Chul Lee |
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| Rok vydání: | 2000 |
| Předmět: |
Physiology
Aminocyclopropanecarboxylate oxidase Molecular Sequence Data Plant Science Biology Polymerase Chain Reaction Gene Expression Regulation Plant Auxin Complementary DNA Gene expression Amino Acid Sequence Gene chemistry.chemical_classification Regulation of gene expression Oxidase test Indoleacetic Acids cDNA library Chromosome Mapping food and beverages Oryza Cell Biology General Medicine Ethylenes Blotting Northern Blotting Southern chemistry Biochemistry RNA Plant Amino Acid Oxidoreductases |
| Zdroj: | Plant and Cell Physiology. 41:354-362 |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/41.3.354 |
| Popis: | Two cDNA clones, pOS-ACO2 and pOS-ACO3, encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase were isolated from rice seedling cDNA library. pOS-ACO3 is a 1,299 bp full-length clone encoding 321 amino acids (Mr=35.9 kDa), while pOS-ACO2 is 1,072 bp long and is a partial cDNA clone encoding 314 amino acids. These two deduced amino acid sequences share 70% identity, and display a high degree of sequence identity (72-92%) with previously isolated pOS-ACO1 of deepwater rice. The chromosomal location studies show that OS-ACO2 is positioned on the long arm of chromosome 9, while OS-ACO3 on the long arm of chromosome 2 of rice genome. A marked increase in the level of OS-ACO2 transcript was observed in IAA-treated etiolated rice seedlings, whereas the OS-ACO3 mRNA was greatly accumulated by ethylene treatment. Results of ethylene inhibitor studies indicated that auxin promotion of the OS-ACO2 transcription was not mediated through the action of auxin-induced ethylene. Thus, it appears that there are two groups of ACC oxidase transcripts in rice plants, either auxin-induced or ethylene-induced. The auxin-induced OS-ACO2 expression was partially inhibited by ethylene, while ethylene induction of OS-ACO3 transcription was completely blocked by auxin. These results indicate that the expression of ACC oxidase genes is regulated by complex hormonal networks in a gene specific manner in rice seedlings. Okadaic acid, a potent inhibitor of protein phosphatase, effectively suppressed the IAA induction of OS-ACO2 expression, suggesting that protein dephosphorylation plays a role in the induction of ACC oxidase by auxin. A scheme of the multiple regulatory pathways for the expression of ACC oxidase gene family by auxin, ethylene and protein phosphatase is presented. |
| Databáze: | OpenAIRE |
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