Curcumin protects rat hippocampal neurons against pseudorabies virus by regulating the BDNF/TrkB pathway
| Autor: | Xianmei Luo, Luqiu Feng, Guodong Luo, Ling Gan, Chen Zhang, Bingjie Yang |
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| Rok vydání: | 2020 |
| Předmět: |
Curcumin
Science viruses animal diseases Apoptosis Tropomyosin receptor kinase B Pharmacology Hippocampal formation Nitric Oxide medicine.disease_cause Antiviral Agents Neuroprotection Antioxidants Article chemistry.chemical_compound Neurotrophic factors medicine Animals Receptor trkB Cells Cultured Membrane Potential Mitochondrial Multidisciplinary biology Superoxide Dismutase Chemistry Brain-Derived Neurotrophic Factor Pyramidal Cells virus diseases biochemical phenomena metabolism and nutrition Herpesvirus 1 Suid Mitochondria Rats Nitric oxide synthase Oxidative Stress Neuroprotective Agents Neurology nervous system biology.protein Medicine Oxidation-Reduction Oxidative stress Signal Transduction Neuroscience |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-78903-0 |
| Popis: | Pseudorabies virus (PRV) infection can elicit nervous system disorders. Curcumin has been reported to have neuroprotective effects. However, whether curcumin can protect neurons against PRV infection and the underlying mechanisms remain unclear. In the present study, for the first time, the protective effects of curcumin against PRV-induced oxidative stress, apoptosis, and mitochondrial dysfunction in rat hippocampal neurons and the brain-derived neurotrophic factor (BDNF)/tropomyosin-related kinase B (TrkB) pathway were investigated. Results indicated that PRV with a titer of 3.06 × 106 TCID50 (50% tissue culture infective dose) induced oxidative damage of hippocampal neurons 2 h post-infection and that 10 μM curcumin improved the viability of PRV-infected hippocampal neurons. Blocking the BDNF/TrkB pathway reversed the neuroprotective effects of curcumin, which were imparted by decreasing the PRV-induced upregulation of nitric oxide synthase expression, repressing the PRV-activated mitochondrial apoptotic pathway, and mitochondrial dysfunction. To conclude, curcumin exhibited a neuroprotective role against PRV infection by upregulating the BDNF/TrkB pathway. This study provides insight into the anti-PRV neuroprotective application of curcumin and the underlying mechanism in the prophylaxis and treatment of neurological disorders caused by PRV infection. |
| Databáze: | OpenAIRE |
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