Expression of multiple human endogenous retrovirus surface envelope proteins in ovarian cancer

Autor: Kirstin F. Barnhart, Kiera Rycaj, Dung Tsa Chen, Daniel G. Rosen, Feng Wang-Johanning, Miao Huang, Jinsong Liu, Kate Tsai, Danielle W. Lu, Gary L. Johanning
Rok vydání: 2006
Předmět:
Adult
Cancer Research
endocrine system diseases
viruses
Molecular Sequence Data
Cell
Fluorescent Antibody Technique
Enzyme-Linked Immunosorbent Assay
Biology
Immunoenzyme Techniques
Gene expression
Tumor Cells
Cultured
medicine
Humans
Amino Acid Sequence
RNA
Messenger
Gene
Aged
Aged
80 and over
Ovarian Neoplasms
Messenger RNA
Base Sequence
Reverse Transcriptase Polymerase Chain Reaction
Endogenous Retroviruses
Ovary
Gene Products
env
Membrane Proteins
Cancer
Middle Aged
Flow Cytometry
medicine.disease
Adenocarcinoma
Mucinous
Virology
Cystadenocarcinoma
Serous
medicine.anatomical_structure
Oncology
Tissue Array Analysis
Case-Control Studies
embryonic structures
Cancer research
biology.protein
Immunohistochemistry
Female
Antibody
Ovarian cancer
Carcinoma
Endometrioid
Adenocarcinoma
Clear Cell
Zdroj: International Journal of Cancer. 120:81-90
ISSN: 1097-0215
0020-7136
Popis: Individual classes of human endogenous retrovirus (HERV) genes and proteins are expressed in cancer, but expression of more than one type of HERV is rare. We report here the expression of multiple HERV genes and proteins in ovarian cell lines and tissues. Expression of HERV-K env mRNA was greater in ovarian epithelial tumors than in normal ovarian tissues (N = 254). The expression of this protein on the surface and in the cytoplasm of ovarian cancer cells was confirmed using anti-HERV-K specific antibody by flow cytometric analysis. The frequency of expression of HERV-K env protein in multitissue microarrays (N = 641) was determined by immunohistochemistry and a significant correlation with tumor histotype was found. A significantly increased expression of HERV-K was observed in tumors with low malignant potential and low grade, relative to expression in normal ovarian tissues. The increase in expression of HERV-K env protein took place in a stepwise fashion in serous papillary adenocarcinoma. Interestingly, we found that other classes of HERV env mRNAs, including ERV3 and HERV-E, are expressed in the same ovarian cancer tissues that expressed HERV-K. Furthermore, anti-HERV antibodies including anti-ERV3 (30%), anti-HERV-E (40%) and anti-HERV-K (55%) were detected in patients with ovarian cancer, but not in normal female controls. HERV env proteins are frequently transcribed and translated in ovarian epithelial tumors, and multiple HERV families are detectable in ovarian cancer. HERV env proteins, and especially those expressed on the cell surface, may serve as novel tumor targets for detection, diagnosis and immunotherapy of ovarian cancer.
Databáze: OpenAIRE
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