Expression of multiple human endogenous retrovirus surface envelope proteins in ovarian cancer
Autor: | Kirstin F. Barnhart, Kiera Rycaj, Dung Tsa Chen, Daniel G. Rosen, Feng Wang-Johanning, Miao Huang, Jinsong Liu, Kate Tsai, Danielle W. Lu, Gary L. Johanning |
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Rok vydání: | 2006 |
Předmět: |
Adult
Cancer Research endocrine system diseases viruses Molecular Sequence Data Cell Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Biology Immunoenzyme Techniques Gene expression Tumor Cells Cultured medicine Humans Amino Acid Sequence RNA Messenger Gene Aged Aged 80 and over Ovarian Neoplasms Messenger RNA Base Sequence Reverse Transcriptase Polymerase Chain Reaction Endogenous Retroviruses Ovary Gene Products env Membrane Proteins Cancer Middle Aged Flow Cytometry medicine.disease Adenocarcinoma Mucinous Virology Cystadenocarcinoma Serous medicine.anatomical_structure Oncology Tissue Array Analysis Case-Control Studies embryonic structures Cancer research biology.protein Immunohistochemistry Female Antibody Ovarian cancer Carcinoma Endometrioid Adenocarcinoma Clear Cell |
Zdroj: | International Journal of Cancer. 120:81-90 |
ISSN: | 1097-0215 0020-7136 |
Popis: | Individual classes of human endogenous retrovirus (HERV) genes and proteins are expressed in cancer, but expression of more than one type of HERV is rare. We report here the expression of multiple HERV genes and proteins in ovarian cell lines and tissues. Expression of HERV-K env mRNA was greater in ovarian epithelial tumors than in normal ovarian tissues (N = 254). The expression of this protein on the surface and in the cytoplasm of ovarian cancer cells was confirmed using anti-HERV-K specific antibody by flow cytometric analysis. The frequency of expression of HERV-K env protein in multitissue microarrays (N = 641) was determined by immunohistochemistry and a significant correlation with tumor histotype was found. A significantly increased expression of HERV-K was observed in tumors with low malignant potential and low grade, relative to expression in normal ovarian tissues. The increase in expression of HERV-K env protein took place in a stepwise fashion in serous papillary adenocarcinoma. Interestingly, we found that other classes of HERV env mRNAs, including ERV3 and HERV-E, are expressed in the same ovarian cancer tissues that expressed HERV-K. Furthermore, anti-HERV antibodies including anti-ERV3 (30%), anti-HERV-E (40%) and anti-HERV-K (55%) were detected in patients with ovarian cancer, but not in normal female controls. HERV env proteins are frequently transcribed and translated in ovarian epithelial tumors, and multiple HERV families are detectable in ovarian cancer. HERV env proteins, and especially those expressed on the cell surface, may serve as novel tumor targets for detection, diagnosis and immunotherapy of ovarian cancer. |
Databáze: | OpenAIRE |
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