| Popis: |
Eighty-seven, 72 and 30 kDa heat-shock proteins from color carp testis cell line, CCT, were purified, and rabbit antibodies were raised against them. After heat shock at 37°C, hsp87 appeared mainly in the cytoplasm and hsp72 and hsp30 appeared in the nucleus. When cells were restressed at 37°C for 8 hr after three recovery periods, the intensity and localization of the three anti-CCT hsps staining changed from those following an initial stress for 8 hr. The anti-CCT hsp87 and hsp72 antibodies crossreacted with proteins of similar molecular weights in all tested fish, lizard, mouse and human cell lines, but they showed various degrees in antigenic relevance. |
