Norcantharidin-associated galactosylated chitosan nanoparticles for hepatocyte-targeted delivery
| Autor: | Wen-Juan Wang, Xue-Nong Zhang, Wei Hu, Jing-Yu Xu, Qiang Zhang, Qin Wang, Yong-Yan Bei, Zhan-Hong Hu, Liang Zhang |
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| Rok vydání: | 2009 |
| Předmět: |
Materials science
Carcinoma Hepatocellular Stereochemistry Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Nanoparticle Bioengineering Chitosan chemistry.chemical_compound Mice In vivo Cell Line Tumor medicine Animals General Materials Science Cytotoxicity Drug Carriers Norcantharidin Liver Neoplasms technology industry and agriculture Galactose Bridged Bicyclo Compounds Heterocyclic In vitro medicine.anatomical_structure chemistry Hepatocyte Molecular Medicine Nanoparticles Particle size Nuclear chemistry |
| Zdroj: | Nanomedicine : nanotechnology, biology, and medicine. 6(2) |
| ISSN: | 1549-9642 |
| Popis: | In this study a new chitosan (CS) derivative, galactosylated chitosan (GC), was synthesized and used to prepare norcantharidin-associated GC nanoparticles (NCTD-GC NPs) by taking advantage of the ionic cross-linkage between the molecules of the anti-hepatocarcinoma medicine NCTD and of the GC as carrier. NCTD-GC NPs were obtained with average particle size of 118.68 ± 3.37 nm, entrapment efficiency of 57.92 ± 0.40%, and drug-loading amount of 10.38 ± 0.06%. Several important factors influencing the entrapment efficiency, drug-loading amount, and particle size of NCTD-GC NPs were studied. The characteristics of sustained and pH-sensitive release of NCTD from NCTD-GC NPs in vitro were studied. In addition, in vitro cellular uptake and cytotoxicity of nanoparticles to hepatoma cell lines SMMC-7721 and HepG2 were also investigated. In vitro, and compared to CS-based NCTD-CS NPs, NCTD-GC NPs demonstrated satisfactory compatibility with hepatoma cells and strong cytotoxicity against hepatocellular carcinoma cells. In vivo antitumor activity of NCTD-GC NPs was evaluated in mice bearing H22 liver tumors. NCTD-GC NPs displayed tumor inhibition effect in mice, better than either the free NCTD or the NCTD-CS NPs. As a hepatocyte-targeting carrier, GC NPs are potentially promising for clinical applications. From the Clinical Editor In this paper, a galactosylated chitosan (GC), was synthesized and norcantharidin (NCTD)-associated galactosylated chitosan nanoparticles (NCTDGC NPs) were generated by coupling NCTD - an anti-hepatocarcinoma drug - and GC as carrier. Compared to chitosan nanoparticles, NCTD-GC-NPs demonstrated satisfactory compatibility with hepatoma cells and strong cytotoxicity against the cells. |
| Databáze: | OpenAIRE |
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