Developing A New Strategy for Delivery of Neural Transplant Populations using Precursor Cell Sprays and Specialised Cell Media
| Autor: | Farhana Chowdhury, William Aidan Woods, Christopher F. Adams, Divya M. Chari, Nikolaos Tzerakis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
traumatic injury
business.industry Cell stem cell spray Cell delivery Q1 R1 Cell biology medicine.anatomical_structure Traumatic injury cell delivery Precursor cell Medical technology General Earth and Planetary Sciences Medicine neural repair aerosolization R855-855.5 business TP248.13-248.65 Aerosolization Biotechnology General Environmental Science |
| Zdroj: | Advanced NanoBiomed Research, Vol 1, Iss 9, Pp n/a-n/a (2021) |
| ISSN: | 2699-9307 |
| Popis: | Neural precursor/stem cell transplantation therapies promote regeneration in neurological injuries, but current cell delivery methods have drawbacks. These include risks with surgical microinjection (e.g., hemorrhage, embolism) and high cell loss with systemic delivery/passage through fine gauge needles. Aerosolized cell delivery offers significant benefits including rapid and minimally invasive cell delivery, and ease of delivery to end users. To develop this approach, it is necessary to prove that 1) aerosolization does not have detrimental effects on transplant cells and 2) suitable media can be identified to support cell delivery. To achieve these aims, cells are sprayed using a commercial spray device or stored in Hibernate‐A, a CO2‐independent nutrient solution. Histological assessments consist of cell viability analysis, immunocytochemistry, and EdU labeling. It is shown that a major neural precursor transplant population—oligodendrocyte precursor cells (OPCs)—survive following aerosolized delivery and retain their capacity for proliferation and differentiation (key to their repair function). Hibernate‐A can support OPCs’ survival without specialized maintenance conditions, with no detrimental impact on cell fate. It is considered that this data supports the concept of a novel class of advanced medical spray devices to facilitate transport and delivery of transplant populations in neural cell therapy. |
| Databáze: | OpenAIRE |
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