Induction of cytosolic glutathione S-transferases from Atlantic eel (Anguilla Anguilla) after intraperitoneal treatment with polychlorinated biphenyls
| Autor: | M.C Nóvoa Valiñas, M. Pérez López, M. J. Melgar Riol |
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| Rok vydání: | 2002 |
| Předmět: |
Gene isoform
Environmental Engineering Protein subunit Environmental pollution Kidney Isozyme chemistry.chemical_compound medicine Environmental Chemistry Animals Waste Management and Disposal Chromatography High Pressure Liquid Glutathione Transferase chemistry.chemical_classification Glutathione Anguilla Pollution Polychlorinated Biphenyls Isoenzymes Cytosol medicine.anatomical_structure Enzyme Biochemistry chemistry Liver Enzyme Induction Environmental Pollutants Injections Intraperitoneal |
| Zdroj: | The Science of the total environment. 297(1-3) |
| ISSN: | 0048-9697 |
| Popis: | Glutathione S-transferases (GST) are phase II biotransformation enzymes influenced by exposure to foreign compounds and are proposed as biomarkers of environmental pollution. The hepatic and renal cytosolic GST isoenzymes from juvenile Atlantic eels were isolated and partially characterized. In both organs, three different GST isoenzymes were purified using HPLC and the final subcellular purified fraction resulted in enzymatic subunits of approximately 24 500 Da. Total GST activity was significantly higher towards the substrate 1-chloro-2,4-dinitrobenzene (CDNB) than the ethacrynic acid (ETHA). Induction of isoenzymes of GST was studied after intraperitoneal treatment with a single dose (25 mg/kg bw) of a polychlorinated biphenyls mixture. In the hepatic cytosol, an inductory effect on the major enzymatic isoform was observed when comparing treated with control eels. This was directly related to statistically significant (P |
| Databáze: | OpenAIRE |
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