Determination of interstitial collagenase (MMP-1) in patients with rheumatoid arthritis
| Autor: | Takashi Sawai, Y Takahashi, Sakurai M, Shingo Maeda, M Uzuki, H Omoto, Masayuki Seki |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Matrix metalloproteinase inhibitor Immunology Arthritis Enzyme-Linked Immunosorbent Assay Matrix metalloproteinase Matrix Metalloproteinase Inhibitors General Biochemistry Genetics and Molecular Biology Extracellular matrix Arthritis Rheumatoid Rheumatology Synovial Fluid medicine Immunology and Allergy Synovial fluid Humans Collagenases Aged Glycoproteins Aged 80 and over business.industry Synovial Membrane Metalloendopeptidases Tissue Inhibitor of Metalloproteinases Middle Aged medicine.disease Immunohistochemistry medicine.anatomical_structure Collagenase Interstitial collagenase Female Synovial membrane Matrix Metalloproteinase 1 business Biomarkers medicine.drug Research Article |
| Zdroj: | Annals of the rheumatic diseases. 54(12) |
| ISSN: | 0003-4967 |
| Popis: | OBJECTIVES--To investigate whether interstitial collagenase (MMP-1) concentration in synovial fluid can be useful as a marker for disease activity in rheumatoid arthritis (RA), to determine the main route by which collagenase degrades the matrix of articular cartilage, and to investigate if an imbalance between metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMP) is responsible for the activity of MMPs in RA. METHODS--Collagenase concentrations were measured in synovial fluid and paired serum samples using a specific sandwich enzyme linked immunosorbent assay. Collagenase activities were also assayed in synovial fluid samples. Synovial tissues obtained from the same patient were examined by immunohistochemical staining and the numbers of cells expressing collagenase were counted. RESULTS--Collagenase concentrations in synovial fluid did not correlate with C reactive protein and collagenase levels in serum, but did correlate positively with the degree of synovial inflammation, and increased with increasing numbers of cells identified as expressing collagenase in synovial tissue. Collagenase activities did not correlate with TIMP-1 concentrations, but did correlate strongly with the ratios of collagenase concentration to TIMP-1 (r = 0.73). CONCLUSION--The collagenase concentration in synovial fluid cannot be used as a marker for systemic disease activity, but can be used as a marker for the degree of synovial inflammation in the joint from which the sample is aspirated. In advanced RA, most of the collagenase is probably produced in synovial lining cells and released into synovial fluid, where it degrades the matrix of articular cartilage. An imbalance between MMP and TIMP may be of importance in the degradation of extracellular matrix of articular cartilage in RA. |
| Databáze: | OpenAIRE |
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