Divergent in vitro/in vivo responses to drug treatments of highly aggressive NIH-Ras cancer cells: A PET imaging and metabolomics-mass-spectrometry study
| Autor: | Daniela Gaglio 1, 2, 6, 8, Silvia Valtorta 1, 3, 4, Marilena Ripamonti 1, Marcella Bonanomi 2, Chiara Damiani 2, Sergio Todde 5, Alfredo Simone Negri 6, Francesca Sanvito 7, Fabrizia Mastroianni 2, Antonella Di Campli 8, Gabriele Turacchio 8, Giuseppe Di Grigoli 1, Sara Belloli 1, Alberto Luini 8, Maria Carla Gilardi 1, Anna Maria Colangelo 2, 9, Lilia Alberghina 2, Rosa Maria Moresco 2 |
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| Přispěvatelé: | Gaglio, D, Valtorta, S, Ripamonti, M, Bonanomi, M, Damiani, C, Todde, S, Negri, A, Sanvito, F, Mastroianni, F, Di Campli, A, Turacchio, G, Di Grigoli, G, Belloli, S, Luini, A, Gilardi, M, Colangelo, A, Alberghina, L, Moresco, R |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology Gerontology medicine.medical_specialty Mice Nude Mass Spectrometry Metabolomics-mass-spectrometry Mice 03 medical and health sciences Metabolomics Internal medicine medicine Tumor metabolic rewaring PET imaging metabolomics-mass-spectrometry oncogenic-K-ras Animals Tumor growth PET-imaging In vitro in vivo Oncogenic-K-ra Tumor business.industry Environmental adaptation Neoplasms Experimental Pet imaging 3. Good health Genes ras 030104 developmental biology Positron-Emission Tomography Cancer metabolism Cancer cell NIH 3T3 Cells oncogenic-K-ras Stress conditions Metabolic rewiring business Glycolysis Research Paper |
| Zdroj: | Oncotarget Oncotarget 7 (2016): 52017–52031. doi:10.18632/oncotarget.10470 info:cnr-pdr/source/autori:Daniela Gaglio 1,2,6,8, Silvia Valtorta 1,2,3,4, Marilena Ripamonti 1,2, Marcella Bonanomi 2, Chiara Damiani 2, Sergio Todde 5, Alfredo Simone Negri 6, Francesca Sanvito 7, Fabrizia Mastroianni 2, Antonella Di Campli 8, Gabriele Turacchio 8, Giuseppe Di Grigoli 1,2,3, Sara Belloli 1,2,3, Alberto Luini 8, Maria Carla Gilardi 1,2, Anna Maria Colangelo 2,9, Lilia Alberghina 2,9,*, Rosa Maria Moresco 2,3,4,*/titolo:Divergent in vitro%2Fin vivo responses to drug treatments of highly aggressive NIH-Ras cancer cells: a PET imaging and metabolomics-mass-spectrometry study./doi:10.18632%2Foncotarget.10470/rivista:Oncotarget/anno:2016/pagina_da:52017/pagina_a:52031/intervallo_pagine:52017–52031/volume:7 |
| DOI: | 10.18632/oncotarget.10470 |
| Popis: | // Daniela Gaglio 1, 2, 6, 8 , Silvia Valtorta 1, 2, 3, 4 , Marilena Ripamonti 1, 2 , Marcella Bonanomi 2 , Chiara Damiani 2 , Sergio Todde 5 , Alfredo Simone Negri 6 , Francesca Sanvito 7 , Fabrizia Mastroianni 2 , Antonella Di Campli 8 , Gabriele Turacchio 8 , Giuseppe Di Grigoli 1, 2, 3 , Sara Belloli 1, 2, 3 , Alberto Luini 8 , Maria Carla Gilardi 1, 2 , Anna Maria Colangelo 2, 9 , Lilia Alberghina 2, 9, * , Rosa Maria Moresco 2, 3, 4, * 1 Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), Segrate, Italy 2 SYSBIO.IT, Centre of Systems Biology, Milano, Italy 3 Experimental Imaging Center, IRCCS San Raffaele Scientific Institute, Milan, Italy 4 Department of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy 5 Tecnomed Foundation of University of Milano-Bicocca, Monza, Italy 6 Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy University of Milan, Milan, Italy 7 Mouse Histopathology Unit, Department of Pathology, IRCCS San Raffaele Scientific Institute, Milan, Italy 8 Institute of Protein Biochemistry, National Research Council, Naples, Italy 9 Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy * These authors have contributed equally to this work Correspondence to: Daniela Gaglio, email: daniela.gaglio@ibfm.cnr.it Rosa Maria Moresco, email: moresco.rosamaria@hsr.it Keywords: tumor, metabolic rewiring, PET-imaging, metabolomics-mass-spectrometry, oncogenic-K-ras Received: February 02, 2016 Accepted: June 17, 2016 Published: July 07, 2016 ABSTRACT Oncogenic K-ras is capable to control tumor growth and progression by rewiring cancer metabolism. In vitro NIH-Ras cells convert glucose to lactate and use glutamine to sustain anabolic processes, but their in vivo environmental adaptation and multiple metabolic pathways activation ability is poorly understood. Here, we show that NIH-Ras cancer cells and tumors are able to coordinate nutrient utilization to support aggressive cell proliferation and survival. Using PET imaging and metabolomics-mass spectrometry, we identified the activation of multiple metabolic pathways such as: glycolysis, autophagy recycling mechanism, glutamine and serine/glycine metabolism, both under physiological and under stress conditions. Finally, differential responses between in vitro and in vivo systems emphasize the advantageous and uncontrolled nature of the in vivo environment, which has a pivotal role in controlling the responses to therapy. |
| Databáze: | OpenAIRE |
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