Comparison of faecal Lactobacillus populations in experimental animals from different breeding facilities and possible consequences for probiotic studies
| Autor: | G.C.A.M. Bokken, R. de Waard, P.S.T. Tan, J. Snel, F. Schut, J.H.J. Huis in 't Veld |
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| Rok vydání: | 2002 |
| Předmět: |
Breeding
Applied Microbiology and Biotechnology DNA Ribosomal law.invention Microbiology Probiotic Feces Mice law Lactobacillus Animals Laboratory RNA Ribosomal 16S Animals Rats Wistar Phylogeny biology Host (biology) Probiotics food and beverages biology.organism_classification Housing Animal Rats Electrophoresis Polyacrylamide Gel 16s rdna analysis Animal facility |
| Zdroj: | Letters in applied microbiology. 34(2) |
| ISSN: | 0266-8254 |
| Popis: | Aims: The effect of probiotic lactobacilli is likely dependent on the indigenous Lactobacillus strains in the intestinal tract. Since a substantial number of probiotic studies is performed in rodents, we compared the Lactobacillus strains of different rat and mouse populations in three animal facilities. Methods and Results: SDS-PAGE and 16S rDNA analysis of cultured faecal lactobacilli revealed that different Lactobacillus strains were detected in genetically similar Wistar rats bred at different locations. Further, within the same animal facility host genetics did not affect the types of the predominant lactobacilli strains. Conclusions: Our results show that the environmental background of laboratory animals rather than host genetics determines the indigenous Lactobacillus strains that are found. Significance and Impact of the Study: Our findings underline the importance of microflora analysis in probiotic studies. |
| Databáze: | OpenAIRE |
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