Enzyme-treated soy protein supplementation in low protein diet enhanced immune function of immune organs in on-growing grass carp
| Autor: | Ling Tang, Yan Song, Liang-Chao Yan, Pei Wu, Wei-Wei Xiao, Yang Liu, Lin Feng, Sheng-Yao Kuang, Xiao-Qiu Zhou, Wei-Dan Jiang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Carps Low protein medicine.medical_treatment Spleen P70-S6 Kinase 1 Aquatic Science Biology Antioxidants Random Allocation 03 medical and health sciences Immune system Low-protein diet Internal medicine medicine Animals Environmental Chemistry Soy protein Dose-Response Relationship Drug 04 agricultural and veterinary sciences General Medicine biology.organism_classification Animal Feed Immunity Innate Diet Grass carp Aeromonas hydrophila 030104 developmental biology medicine.anatomical_structure Endocrinology Dietary Supplements Soybean Proteins 040102 fisheries 0401 agriculture forestry and fisheries Dietary Proteins |
| Zdroj: | Fish & Shellfish Immunology. 106:318-331 |
| ISSN: | 1050-4648 |
| Popis: | A 56 days feeding trial was conducted to investigate the effects of enzyme-treated soy protein (ETSP) supplementation in low protein diets on immune function of immune organs (head kidney, spleen and skin) in on-growing grass carp. A total of 540 on-growing grass carp (initial average weight: 325.72 ± 0.60 g) were fed six diets, which included a normal protein diet (28% crude protein) and five low protein diets (26% crude protein) supplemented with graded levels of ETSP (0.0, 0.8, 1.2, 1.6 and 2.0%). At the end of feeding period, a challenge test was performed by infection with Aeromonas hydrophila for two weeks. The results indicated that (1) reducing dietary protein content from 28 to 26% decreased antibacterial substances and aggravated inflammatory responses of above three immune organs; (2) under the condition of reducing protein level in diet, 0.8–1.2% ETSP supplementation reversed these above adverse effects on immune function of above three immune organs; (3) suitable ETSP supplementation-decreased inflammatory responses were partly associated with [IκB kinase β (IKKβ)/inhibitor of κBα (IκBα)/nuclear factor kappa B (NF-κB) p65 and p52 or NF-κB p65] signaling and [target of rapamycin (TOR)/(S6K1, 4E-BP)] signaling in above three immune organs. (4) On the basis of C3 content (head kidney), C4 content (spleen) and skin hemorrhage and lesion, the optimal ETSP supplementation levels in low protein diets were estimated to be 1.48%, 1.61% and 1.03%, respectively. In summary, ETSP supplementation in low protein diets improved immune function of head kidney, spleen and skin in on-growing grass carp. |
| Databáze: | OpenAIRE |
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