Fibromodulin Expression in Folliculostellate Cells and Pericytes Is Promoted by TGFβ Signaling in Rat Anterior Pituitary Gland
| Autor: | Kotaro Horiguchi, Rahimi Syaidah, Ken Fujiwara, Morio Azuma, Motoshi Kikuchi, Takehiro Tsukada, Takashi Yashiro |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty fibromodulin Histology Physiology Immunocytochemistry In situ hybridization Biochemistry Pathology and Forensic Medicine Extracellular matrix 03 medical and health sciences Anterior pituitary Internal medicine pericyte medicine Receptor small leucine-rich proteoglycans transforming growth factor beta Messenger RNA biology Chemistry Regular Article Cell Biology Transforming growth factor beta folliculostellate cell Cell biology 030104 developmental biology medicine.anatomical_structure Endocrinology biology.protein Pericyte |
| Zdroj: | Acta Histochemica et Cytochemica |
| ISSN: | 1347-5800 0044-5991 |
| Popis: | Fibromodulin belongs to the family of small leucine-rich proteoglycans (SLRPs), an active component of extracellular matrix. It directly binds collagens to promote fibrillogenesis and also binds transforming growth factor-beta (TGFβ) to antagonize its actions. Our previous studies of rat anterior pituitary gland revealed that fibromodulin is expressed in folliculostellate cells and pericytes. Although our recent study showed that TGFβ2 secreted from folliculostellate cells induces collagen synthesis in pericytes, the involvement of fibromodulin in TGFβ2-mediated collagen regulation has not been studied. The present study examined the effect of TGFβ2 on fibromodulin synthesis in rat anterior pituitary gland. In situ hybridization for TGFβ receptor II and immunohistological techniques revealed the presence of TGFβ receptor II in folliculostellate cells and pericytes. To confirm canonical TGFβ intracellular signaling, Smad2 immunocytochemistry was performed. Nuclear translocation of Smad2 was observed in folliculostellate cells and pericytes after TGFβ2 treatment. TGFβ2 strongly enhanced fibromodulin mRNA and protein expressions, and TGFβ2-induced mRNA expression was completely blocked by TGFβ receptor I inhibitor (SB431542). These results suggest that folliculostellate cells and pericytes exhibit canonical TGFβ2 signaling, which is associated with fibromodulin production. Thus, this is the first report to show that TGFβ signaling regulates the endogenous TGFβ antagonist fibromodulin in the gland. |
| Databáze: | OpenAIRE |
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