Duality of β-glucan microparticles: antigen carrier and immunostimulants
| Autor: | Kim Baert, Bert Devriendt, Bruno G. De Geest, Henri De Greve, Eric Cox |
|---|---|
| Přispěvatelé: | Structural Biology Brussels, Department of Bio-engineering Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
beta-Glucans T-Lymphocytes Sus scrofa Pharmaceutical Science VACCINE Plasma protein binding law.invention COMPLEMENT International Journal of Nanomedicine law Drug Discovery BINDING Medicine and Health Sciences Receptor Original Research PIGLETS chemistry.chemical_classification β-glucan microparticles Escherichia coli Proteins General Medicine IMMUNIZATION Receptors Complement antigen delivery vehicle Recombinant DNA Protein Binding FIMBRIAL ADHESIN FEDF COLI Protein subunit Biophysics Bioengineering Biology Microbiology Biomaterials Immunomodulation 03 medical and health sciences DELIVERY immunostimulants Immune system Antigen Adjuvants Immunologic Animals Antigens Adhesins Bacterial Glucan Cell Proliferation Reactive oxygen species Organic Chemistry Protein Subunits 030104 developmental biology chemistry CELLS FedF SYSTEM |
| Zdroj: | INTERNATIONAL JOURNAL OF NANOMEDICINE International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| DOI: | 10.2147/ijn.s101881 |
| Popis: | Kim Baert,1 Bruno G De Geest,2 Henri De Greve,3,4 Eric Cox,1,* Bert Devriendt1,* 1Department of Virology, Parasitology and Immunology, 2Department of Pharmaceutics, Ghent University, Merelbeke, Ghent, Belgium; 3Structural Biology Research Centre, VIB,Brussels, Belgium; 4Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium *These authors contributed equally tothis work Abstract: Designing efficient recombinant mucosal vaccines against enteric diseases is still a major challenge. Mucosal delivery of recombinant vaccines requires encapsulation in potent immunostimulatory particles to induce an efficient immune response. This paper evaluates the capacity of β-glucan microparticles (GPs) as antigen vehicles and characterizes their immune-stimulatory effects. The relevant infectious antigen FedF was chosen to be loaded inside the microparticles. The incorporation of FedF inside the particles was highly efficient (roughly85%) and occurred without antigen degradation. In addition, these GPs have immunostimulatory effects as well, demonstrated by the strong reactive oxygen species (ROS) production by porcine neutrophils upon their recognition. Although antigen-loaded GPs still induce ROS production, antigen loading decreases this production by neutrophils for reasons yet unknown. However, these antigen-loaded GPs are still able to bind their specific β-glucan receptor, demonstrated by blocking complement receptor 3, which is the major β-glucan receptor on porcine neutrophils. The dual character of these particles is confirmed by a T-cell proliferation assay. FedF-loaded particles induce a significantly higher FedF-specific T-cell proliferation than soluble FedF. Taken together, these results show that GPs are efficient antigen carriers with immune-stimulatory properties. Keywords: β-glucan microparticles, FedF, antigen delivery vehicle, immunostimulants |
| Databáze: | OpenAIRE |
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